Hydroxytyrosol influences exercise-induced mitochondrial respiratory complex assembly into supercomplexes in rats
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Casuso, Rafael A.; Al Fazazi, Saad; Hidalgo Gutiérrez, Agustín; López García, Luis Carlos; Plaza Díaz, Julio; Rueda Robles, Ascensión; Rodríguez Huertas, Jesús FranciscoEditorial
Elsevier
Fecha
2019Resumen
Hydroxytyrosol (HT) has been demonstrated to improve mitochondrial function, both in sedentary and in exercised
animals. Herein, we assessed the effects of two different doses of HT on exercise-induced mitochondrial
respiratory complex (C) assembly into supercomplexes (SCs) and the relation of the potential results to OPA1
levels and oxidative stress. Wistar rats were allocated into six groups: sedentary (SED), sedentary consuming
20 mg/kg/d of HT (SED-20), sedentary consuming 300 mg/kg/d of HT (SED-300); exercised (EXE), exercised
consuming 20 mg/kg/d of HT (EXE-20) and exercised consuming 300 mg/kg/d of HT (EXE-300). Animals were
exercised and/or supplemented for 10 weeks, and assembly of SCs, mitochondrial oxidative status and expression
of OPA1 were quantified in the gastrocnemius muscle. Both EXE and EXE-20 animals exhibited increased
assembly of CI into SCs, but this effect was absent in EXE-300 animals. Levels of CIII2 assembled into SCs
were only increased in EXE-20 animals. Notably EXE-300 animals showed a decreased relative expression of s-
OPA1 isoforms. Therefore, HT exerted dose-dependent effects on SC assembly in exercised animals. Although the
mechanisms leading to SCs assembly in response to exercise and HT are unclear, it seems that a high HT dose can
prevent SCs assembly during exercise by decreasing the expression of the s-OPA1 isoforms.