@misc{10481/88894,
year = {2019},
url = {https://hdl.handle.net/10481/88894},
abstract = {Hydroxytyrosol (HT) has been demonstrated to improve mitochondrial function, both in sedentary and in exercised
animals. Herein, we assessed the effects of two different doses of HT on exercise-induced mitochondrial
respiratory complex (C) assembly into supercomplexes (SCs) and the relation of the potential results to OPA1
levels and oxidative stress. Wistar rats were allocated into six groups: sedentary (SED), sedentary consuming
20 mg/kg/d of HT (SED-20), sedentary consuming 300 mg/kg/d of HT (SED-300); exercised (EXE), exercised
consuming 20 mg/kg/d of HT (EXE-20) and exercised consuming 300 mg/kg/d of HT (EXE-300). Animals were
exercised and/or supplemented for 10 weeks, and assembly of SCs, mitochondrial oxidative status and expression
of OPA1 were quantified in the gastrocnemius muscle. Both EXE and EXE-20 animals exhibited increased
assembly of CI into SCs, but this effect was absent in EXE-300 animals. Levels of CIII2 assembled into SCs
were only increased in EXE-20 animals. Notably EXE-300 animals showed a decreased relative expression of s-
OPA1 isoforms. Therefore, HT exerted dose-dependent effects on SC assembly in exercised animals. Although the
mechanisms leading to SCs assembly in response to exercise and HT are unclear, it seems that a high HT dose can
prevent SCs assembly during exercise by decreasing the expression of the s-OPA1 isoforms.},
publisher = {Elsevier},
title = {Hydroxytyrosol influences exercise-induced mitochondrial respiratory complex assembly into supercomplexes in rats},
doi = {10.1016/j.freeradbiomed.2019.01.027},
author = {Casuso, Rafael A. and Al Fazazi, Saad and Hidalgo Gutiérrez, Agustín and López García, Luis Carlos and Plaza Díaz, Julio and Rueda Robles, Ascensión and Rodríguez Huertas, Jesús Francisco},
}