Identification of Trypanosomatids by detecting Single Nucleotide Fingerprints using DNA analysis by dynamic chemistry with MALDI-ToF

Luque-González, María Angélica; Sánchez Martín, Rosario María; Ortiz González, Matilde; Soriano Rodríguez, Miguel; García Salcedo, José Antonio; Díaz Mochón, Juan José

doi:10.1016/j.talanta.2017.07.059

Talanta2018_LuqueGonzalez_1-s2.0-S0039914017307816-main.pdf (1.057Mb)

Identificadores

URI: https://hdl.handle.net/10481/88617

DOI: 10.1016/j.talanta.2017.07.059

Exportar

Editorial

Elsevier

Fecha

2017-07-21

Referencia bibliográfica

María Angélica Luque-González, Mavys Tabraue-Chávez, Bárbara López-Longarela, Rosario María Sánchez-Martín, Matilde Ortiz-González, Miguel Soriano-Rodríguez, José Antonio García-Salcedo, Salvatore Pernagallo, Juan José Díaz-Mochón, Identification of Trypanosomatids by detecting Single Nucleotide Fingerprints using DNA analysis by dynamic chemistry with MALDI-ToF, Talanta, Volume 176, 2018, Pages 299-307, ISSN 0039-9140, https://doi.org/10.1016/j.talanta.2017.07.059.

Patrocinador

This research work has received funding from Junta de Andalucía, Consejería de Economía e Innovación (project number 2012- BIO1778), the Spanish Ministerio de Economía y Competitividad (Grants CTQ2012-34778, BIO2016-80519-R, FPI Grant BES-2013- 063020). This research was partially supported by the 7th European Community Framework Program (FP7-PEOPLE-2012-CIG-Project Number 322276). The authors thank DestiNA Genomica S.L. for assistance during the process of isolation of the genomic DNA, PCR amplification and dynamic chemistry reaction. We also thank Carlos Rodrigues-Poveda for technical advice and Mr. Hugh Ilyine for proof-reading.

Resumen

Protozoan parasites of the Trypanosomatidae family cause severe diseases like Human African Trypanosomiasis, Chagas disease, and Leishmaniasis. Despite existing molecular assays for detection and monitoring, limitations persist. We introduce a novel molecular assay combining dynamic chemistry DNA analysis with MALDI-ToF mass spectrometry, allowing rapid identification and differentiation of Trypanosomatid species with singleplex PCR. This innovative approach enhances diagnostic capabilities and therapeutic monitoring for these diseases.

Colecciones

CTS987 - Artículos

Excepto si se señala otra cosa, la licencia del ítem se describe como Attribution-NonCommercial-NoDerivatives 4.0 Internacional