@misc{10481/88617,
year = {2017},
month = {7},
url = {https://hdl.handle.net/10481/88617},
abstract = {Protozoan parasites of the Trypanosomatidae family cause severe diseases like Human African Trypanosomiasis, Chagas disease, and Leishmaniasis. Despite existing molecular assays for detection and monitoring, limitations persist. We introduce a novel molecular assay combining dynamic chemistry DNA analysis with MALDI-ToF mass spectrometry, allowing rapid identification and differentiation of Trypanosomatid species with singleplex PCR. This innovative approach enhances diagnostic capabilities and therapeutic monitoring for these diseases.},
organization = {This research work has received funding from Junta de Andalucía,
Consejería de Economía e Innovación (project number 2012-
BIO1778), the Spanish Ministerio de Economía y Competitividad
(Grants CTQ2012-34778, BIO2016-80519-R, FPI Grant BES-2013-
063020). This research was partially supported by the 7th European
Community Framework Program (FP7-PEOPLE-2012-CIG-Project
Number 322276).
The authors thank DestiNA Genomica S.L. for assistance during the
process of isolation of the genomic DNA, PCR amplification and
dynamic chemistry reaction. We also thank Carlos Rodrigues-Poveda
for technical advice and Mr. Hugh Ilyine for proof-reading.},
publisher = {Elsevier},
title = {Identification of Trypanosomatids by detecting Single Nucleotide Fingerprints using DNA analysis by dynamic chemistry with MALDI-ToF},
doi = {10.1016/j.talanta.2017.07.059},
author = {Luque-González, María Angélica and Sánchez Martín, Rosario María and Ortiz González, Matilde and Soriano Rodríguez, Miguel and García Salcedo, José Antonio and Díaz Mochón, Juan José},
}