Identification of Trypanosomatids by detecting Single Nucleotide Fingerprints using DNA analysis by dynamic chemistry with MALDI-ToF
| dc.contributor.author | Luque-González, María Angélica | |
| dc.contributor.author | Sánchez Martín, Rosario María | |
| dc.contributor.author | Ortiz González, Matilde | |
| dc.contributor.author | Soriano Rodríguez, Miguel | |
| dc.contributor.author | García Salcedo, José Antonio | |
| dc.contributor.author | Díaz Mochón, Juan José | |
| dc.date.accessioned | 2024-02-07T13:26:34Z | |
| dc.date.available | 2024-02-07T13:26:34Z | |
| dc.date.issued | 2017-07-21 | |
| dc.identifier.citation | María Angélica Luque-González, Mavys Tabraue-Chávez, Bárbara López-Longarela, Rosario María Sánchez-Martín, Matilde Ortiz-González, Miguel Soriano-Rodríguez, José Antonio García-Salcedo, Salvatore Pernagallo, Juan José Díaz-Mochón, Identification of Trypanosomatids by detecting Single Nucleotide Fingerprints using DNA analysis by dynamic chemistry with MALDI-ToF, Talanta, Volume 176, 2018, Pages 299-307, ISSN 0039-9140, https://doi.org/10.1016/j.talanta.2017.07.059. | es_ES |
| dc.identifier.uri | https://hdl.handle.net/10481/88617 | |
| dc.description.abstract | Protozoan parasites of the Trypanosomatidae family cause severe diseases like Human African Trypanosomiasis, Chagas disease, and Leishmaniasis. Despite existing molecular assays for detection and monitoring, limitations persist. We introduce a novel molecular assay combining dynamic chemistry DNA analysis with MALDI-ToF mass spectrometry, allowing rapid identification and differentiation of Trypanosomatid species with singleplex PCR. This innovative approach enhances diagnostic capabilities and therapeutic monitoring for these diseases. | es_ES |
| dc.description.sponsorship | This research work has received funding from Junta de Andalucía, Consejería de Economía e Innovación (project number 2012- BIO1778), the Spanish Ministerio de Economía y Competitividad (Grants CTQ2012-34778, BIO2016-80519-R, FPI Grant BES-2013- 063020). This research was partially supported by the 7th European Community Framework Program (FP7-PEOPLE-2012-CIG-Project Number 322276). The authors thank DestiNA Genomica S.L. for assistance during the process of isolation of the genomic DNA, PCR amplification and dynamic chemistry reaction. We also thank Carlos Rodrigues-Poveda for technical advice and Mr. Hugh Ilyine for proof-reading. | es_ES |
| dc.language.iso | eng | es_ES |
| dc.publisher | Elsevier | es_ES |
| dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
| dc.title | Identification of Trypanosomatids by detecting Single Nucleotide Fingerprints using DNA analysis by dynamic chemistry with MALDI-ToF | es_ES |
| dc.type | journal article | es_ES |
| dc.rights.accessRights | embargoed access | es_ES |
| dc.identifier.doi | 10.1016/j.talanta.2017.07.059 | |
| dc.type.hasVersion | VoR | es_ES |
Fichier(s) constituant ce document
![[PDF]](/themes/Mirage2/images/thumbnails/mimes/pdf.png "pdf")
Ce document figure dans la(les) collection(s) suivante(s)
Excepté là où spécifié autrement, la license de ce document est décrite en tant que Attribution-NonCommercial-NoDerivatives 4.0 Internacional