Direct Detection of miR-122 in Hepatotoxicity Using Dynamic Chemical Labeling Overcomes Stability and isomiR Challenges
Metadatos
Mostrar el registro completo del ítemAutor
López-Longarela, B; Morrison, EE; Tranter, JD; Chahman-Vos, L; Léonard, JF; Gautier, JC; Laurent, S; Lartigau, A; Boitier, E; Sautier, L; Carmona-Saez, P; Martorell-Marugan, J; Mellanby, RJ; Pernagallo, S; Ilyine, H; Rissin, DM; Duffy, DC; Dear, JW; Diaz Mochon, Juan JoséEditorial
ACS Publications
Fecha
2020-02-18Referencia bibliográfica
López-Longarela B, Morrison EE, Tranter JD, Chahman-Vos L, Léonard JF, Gautier JC, Laurent S, Lartigau A, Boitier E, Sautier L, Carmona-Saez P, Martorell-Marugan J, Mellanby RJ, Pernagallo S, Ilyine H, Rissin DM, Duffy DC, Dear JW, Díaz-Mochón JJ. Direct Detection of miR-122 in Hepatotoxicity Using Dynamic Chemical Labeling Overcomes Stability and isomiR Challenges. Anal Chem. 2020 Feb 18;92(4):3388-3395. doi: 10.1021/acs.analchem.9b05449. Epub 2020 Jan 27. PMID: 31939284.
Patrocinador
J.D.T. was supported by a Ph.D. studentship from TheCunningham Trust. J.W.D. was supported by an NHSResearch Scotland (NRS) Career Research Fellowship throughNHS Lothian and acknowledges the contribution of the BritishHeart Foundation Centre of Research Excellence Award (RE/08/001). This research was partially supported by the Ministryof Economy and Competitiveness, Spain (BIO2016-80519-R)and Health Insitute Carlos III (ISCIII) (Projects oftechnological development in health - ref DTS18/00121).Resumen
Circulating microRNAs serve as stable and cross-species biomarkers. MicroRNA-122 (miR-122) is a reliable hepatocyte-specific biomarker for drug-induced liver injury (DILI). We developed a dynamic chemical labeling (DCL) assay enabling direct detection of miR-122 in blood, with high specificity and sensitivity from small sample volumes. This assay accurately identifies DILI in humans and animals, offering improved stability and isomiR detection compared to PCR methods.