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Direct Detection of miR-122 in Hepatotoxicity Using Dynamic Chemical Labeling Overcomes Stability and isomiR Challenges

dc.contributor.authorLópez-Longarela, B
dc.contributor.authorMorrison, EE
dc.contributor.authorTranter, JD
dc.contributor.authorChahman-Vos, L
dc.contributor.authorLéonard, JF
dc.contributor.authorGautier, JC
dc.contributor.authorLaurent, S
dc.contributor.authorLartigau, A
dc.contributor.authorBoitier, E
dc.contributor.authorSautier, L
dc.contributor.authorCarmona-Saez, P
dc.contributor.authorMartorell-Marugan, J
dc.contributor.authorMellanby, RJ
dc.contributor.authorPernagallo, S
dc.contributor.authorIlyine, H
dc.contributor.authorRissin, DM
dc.contributor.authorDuffy, DC
dc.contributor.authorDear, JW
dc.contributor.authorDiaz Mochon, Juan José 
dc.date.accessioned2024-02-07T13:22:31Z
dc.date.available2024-02-07T13:22:31Z
dc.date.issued2020-02-18
dc.identifier.citationLópez-Longarela B, Morrison EE, Tranter JD, Chahman-Vos L, Léonard JF, Gautier JC, Laurent S, Lartigau A, Boitier E, Sautier L, Carmona-Saez P, Martorell-Marugan J, Mellanby RJ, Pernagallo S, Ilyine H, Rissin DM, Duffy DC, Dear JW, Díaz-Mochón JJ. Direct Detection of miR-122 in Hepatotoxicity Using Dynamic Chemical Labeling Overcomes Stability and isomiR Challenges. Anal Chem. 2020 Feb 18;92(4):3388-3395. doi: 10.1021/acs.analchem.9b05449. Epub 2020 Jan 27. PMID: 31939284.es_ES
dc.identifier.urihttps://hdl.handle.net/10481/88616
dc.description.abstractCirculating microRNAs serve as stable and cross-species biomarkers. MicroRNA-122 (miR-122) is a reliable hepatocyte-specific biomarker for drug-induced liver injury (DILI). We developed a dynamic chemical labeling (DCL) assay enabling direct detection of miR-122 in blood, with high specificity and sensitivity from small sample volumes. This assay accurately identifies DILI in humans and animals, offering improved stability and isomiR detection compared to PCR methods.es_ES
dc.description.sponsorshipJ.D.T. was supported by a Ph.D. studentship from TheCunningham Trust. J.W.D. was supported by an NHSResearch Scotland (NRS) Career Research Fellowship throughNHS Lothian and acknowledges the contribution of the BritishHeart Foundation Centre of Research Excellence Award (RE/08/001). This research was partially supported by the Ministryof Economy and Competitiveness, Spain (BIO2016-80519-R)and Health Insitute Carlos III (ISCIII) (Projects oftechnological development in health - ref DTS18/00121).es_ES
dc.language.isoenges_ES
dc.publisherACS Publicationses_ES
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleDirect Detection of miR-122 in Hepatotoxicity Using Dynamic Chemical Labeling Overcomes Stability and isomiR Challengeses_ES
dc.typejournal articlees_ES
dc.rights.accessRightsembargoed accesses_ES
dc.identifier.doi10.1021/acs.analchem.9b05449


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