Moving into the red – a near infra-red optical probe for analysis of human neutrophil elastase in activated neutrophils and neutrophil extracellular traps
Metadatos
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Royal Society of Chemistry
Date
2023-09Referencia bibliográfica
Chem. Commun., 2023, 59, 11660[DOI: 10.1039/d3cc03634k]
Patrocinador
UK Research & Innovation (UKRI) Engineering & Physical Sciences Research Council (EPSRC) EP/R005257/1; UK Research & Innovation (UKRI) Medical Research Council UK (MRC) MR/N02995X/1Résumé
Neutrophils are the first immune cells recruited for defence against invading pathogens; however, their dysregulated activation and subsequent release of the enzyme human neutrophil elastase is associated with several, inflammation-based, diseases. Herein, we describe a FRET-based, tri-branched (one quencher, three fluorophores) near infrared probe that provides an intense OFF/ON amplified fluorescence signal for specific detection of human neutrophil elastase. The probe allowed selective detection of activated neutrophils and labelling of neutrophil extracellular traps.
A tri-branched, FRET-based probe for the detection of hNE was synthesised with emission in the NIR region of the spectrum, where endogenous biomolecules have decreased absorbance, and minimal autofluorescence