@misc{10481/85243,
year = {2023},
month = {9},
url = {https://hdl.handle.net/10481/85243},
abstract = {Neutrophils are the first immune cells recruited for defence against invading pathogens; however, their dysregulated activation and subsequent release of the enzyme human neutrophil elastase is associated with several, inflammation-based, diseases. Herein, we describe a FRET-based, tri-branched (one quencher, three fluorophores) near infrared probe that provides an intense OFF/ON amplified fluorescence signal for specific detection of human neutrophil elastase. The probe allowed selective detection of activated neutrophils and labelling of neutrophil extracellular traps.

A tri-branched, FRET-based probe for the detection of hNE was synthesised with emission in the NIR region of the spectrum, where endogenous biomolecules have decreased absorbance, and minimal autofluorescence},
organization = {UK Research & Innovation (UKRI)
Engineering & Physical Sciences Research Council (EPSRC)	EP/R005257/1},
organization = {UK Research & Innovation (UKRI)
Medical Research Council UK (MRC)	MR/N02995X/1},
publisher = {Royal Society of Chemistry},
title = {Moving into the red – a near infra-red optical probe for analysis of human neutrophil elastase in activated neutrophils and neutrophil extracellular traps},
doi = {10.1039/d3cc03634k},
author = {Rodriguez Rios, Maria and Megía Fernández, Alicia},
}