A Standardized Extract of Lentinula edodes Cultured Mycelium Inhibits Pseudomonas aeruginosa Infectivity Mechanisms
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AuthorTena Garitaonaindia, Mireia; Ceacero Heras, Diego; Maldonado Montoro, María del Mar; Sánchez De Medina López-Huertas, Fermín; Martínez Augustín, María Olga; Daddaoua, Abdelali
PrebioticAHCCPseudomonas aeruginosaMotility and biofilmSecretion system and adhesionImmune responsePCR real time (qPCR)Internalization
Tena-Garitaonaindia M... [et al.] (2022) A Standardized Extract of Lentinula edodes Cultured Mycelium Inhibits Pseudomonas aeruginosa Infectivity Mechanisms. Front. Microbiol. 13:814448. doi: [10.3389/fmicb.2022.814448]
SponsorshipFEDER project of Junta de Andalucia, Spain 30B572F301; Ministry of Economy and Competitivity; European Commission SAF2017-88457-R AGL2017-85270-R; University of the Ministry of Education (Spain); Junta de Andalucia CTS235 CTS164; Instituto de Salud Carlos III European Commission
The priority pathogen list of the World Health Organization classified Pseudomonas aeruginosa as the second top critical pathogen. Hence, the development of novel antibacterial strategies to tackle this bacterium is highly necessary. Herein we explore the potential antibacterial effect of a standardized extract of cultured mycelium of Lentinula edodes (AHCCR ) on P. aeruginosa. AHCCR was found to inhibit the growth rate and biofilm formation of strain PAO1. No change in swarming was observed, but AHCCR hampered swimming and twitching motility. In accordance, a decreased expression of metabolism, growth, and biofilm formation genes was shown. AHCCR also diminished the levels of exotoxin A and bacteria inside IEC18 cells and the secretion of IL-6, IL-10 and TNF by infected macrophages. This effect was related to a reduced phosphorylation of MAPKs and to bacteria internalization. Taken together, our data suggest that AHCCR has a potential role to prevent P. aeruginosa infections and may lead to the development of new therapies.