A Standardized Extract of Lentinula edodes Cultured Mycelium Inhibits Pseudomonas aeruginosa Infectivity Mechanisms
Metadatos
Mostrar el registro completo del ítemAutor
Tena Garitaonaindia, Mireia; Ceacero Heras, Diego; Maldonado Montoro, María del Mar; Sánchez De Medina López-Huertas, Fermín; Martínez Augustín, María Olga; Daddaoua, AbdelaliEditorial
Frontiers
Materia
Prebiotic AHCC Pseudomonas aeruginosa Motility and biofilm Secretion system and adhesion Immune response PCR real time (qPCR) Internalization
Fecha
2022-03-16Referencia bibliográfica
Tena-Garitaonaindia M... [et al.] (2022) A Standardized Extract of Lentinula edodes Cultured Mycelium Inhibits Pseudomonas aeruginosa Infectivity Mechanisms. Front. Microbiol. 13:814448. doi: [10.3389/fmicb.2022.814448]
Patrocinador
FEDER project of Junta de Andalucia, Spain 30B572F301; Ministry of Economy and Competitivity; European Commission SAF2017-88457-R AGL2017-85270-R; University of the Ministry of Education (Spain); Junta de Andalucia CTS235 CTS164; Instituto de Salud Carlos III European CommissionResumen
The priority pathogen list of the World Health Organization classified Pseudomonas
aeruginosa as the second top critical pathogen. Hence, the development of novel
antibacterial strategies to tackle this bacterium is highly necessary. Herein we explore the
potential antibacterial effect of a standardized extract of cultured mycelium of Lentinula
edodes (AHCCR
) on P. aeruginosa. AHCCR
was found to inhibit the growth rate and
biofilm formation of strain PAO1. No change in swarming was observed, but AHCCR
hampered swimming and twitching motility. In accordance, a decreased expression of
metabolism, growth, and biofilm formation genes was shown. AHCCR
also diminished
the levels of exotoxin A and bacteria inside IEC18 cells and the secretion of IL-6, IL-10
and TNF by infected macrophages. This effect was related to a reduced phosphorylation
of MAPKs and to bacteria internalization. Taken together, our data suggest that AHCCR
has a potential role to prevent P. aeruginosa infections and may lead to the development
of new therapies.