@misc{10481/74844,
year = {2022},
month = {3},
url = {http://hdl.handle.net/10481/74844},
abstract = {The priority pathogen list of the World Health Organization classified Pseudomonas
aeruginosa as the second top critical pathogen. Hence, the development of novel
antibacterial strategies to tackle this bacterium is highly necessary. Herein we explore the
potential antibacterial effect of a standardized extract of cultured mycelium of Lentinula
edodes (AHCCR
) on P. aeruginosa. AHCCR
 was found to inhibit the growth rate and
biofilm formation of strain PAO1. No change in swarming was observed, but AHCCR

hampered swimming and twitching motility. In accordance, a decreased expression of
metabolism, growth, and biofilm formation genes was shown. AHCCR
 also diminished
the levels of exotoxin A and bacteria inside IEC18 cells and the secretion of IL-6, IL-10
and TNF by infected macrophages. This effect was related to a reduced phosphorylation
of MAPKs and to bacteria internalization. Taken together, our data suggest that AHCCR

has a potential role to prevent P. aeruginosa infections and may lead to the development
of new therapies.},
organization = {FEDER project of Junta de Andalucia, Spain	30B572F301},
organization = {Ministry of Economy and Competitivity},
organization = {European Commission	SAF2017-88457-R	
AGL2017-85270-R},
organization = {University of the Ministry of Education (Spain)},
organization = {Junta de Andalucia	CTS235	
CTS164},
organization = {Instituto de Salud Carlos III
European Commission},
publisher = {Frontiers},
keywords = {Prebiotic},
keywords = {AHCC},
keywords = {Pseudomonas aeruginosa},
keywords = {Motility and biofilm},
keywords = {Secretion system and adhesion},
keywords = {Immune response},
keywords = {PCR real time (qPCR)},
keywords = {Internalization},
title = {A Standardized Extract of Lentinula edodes Cultured Mycelium Inhibits Pseudomonas aeruginosa Infectivity Mechanisms},
doi = {10.3389/fmicb.2022.814448},
author = {Tena Garitaonaindia, Mireia and Ceacero Heras, Diego and Maldonado Montoro, María del Mar and Sánchez De Medina López-Huertas, Fermín and Martínez Augustín, María Olga and Daddaoua, Abdelali},
}