A Standardized Extract of Lentinula edodes Cultured Mycelium Inhibits Pseudomonas aeruginosa Infectivity Mechanisms Tena Garitaonaindia, Mireia Ceacero Heras, Diego Maldonado Montoro, María del Mar Sánchez De Medina López-Huertas, Fermín Martínez Augustín, María Olga Daddaoua, Abdelali Prebiotic AHCC Pseudomonas aeruginosa Motility and biofilm Secretion system and adhesion Immune response PCR real time (qPCR) Internalization This work was supported by grants from FEDER project of Junta de Andalucia, Spain (30B572F301), Ministry of Economy and Competitivity, partly with Fondo Europeo de Desarrollo Regional FEDER funds (SAF2017-88457-R and AGL2017-85270-R), and by Junta de Andalucia (CTS235 and CTS164). MT-G was supported by the University of the Ministry of Education (Spain). CIBERehd is funded by Instituto de Salud Carlos III. The priority pathogen list of the World Health Organization classified Pseudomonas aeruginosa as the second top critical pathogen. Hence, the development of novel antibacterial strategies to tackle this bacterium is highly necessary. Herein we explore the potential antibacterial effect of a standardized extract of cultured mycelium of Lentinula edodes (AHCCR ) on P. aeruginosa. AHCCR was found to inhibit the growth rate and biofilm formation of strain PAO1. No change in swarming was observed, but AHCCR hampered swimming and twitching motility. In accordance, a decreased expression of metabolism, growth, and biofilm formation genes was shown. AHCCR also diminished the levels of exotoxin A and bacteria inside IEC18 cells and the secretion of IL-6, IL-10 and TNF by infected macrophages. This effect was related to a reduced phosphorylation of MAPKs and to bacteria internalization. Taken together, our data suggest that AHCCR has a potential role to prevent P. aeruginosa infections and may lead to the development of new therapies. 2022-05-16T08:37:37Z 2022-05-16T08:37:37Z 2022-03-16 info:eu-repo/semantics/article Tena-Garitaonaindia M... [et al.] (2022) A Standardized Extract of Lentinula edodes Cultured Mycelium Inhibits Pseudomonas aeruginosa Infectivity Mechanisms. Front. Microbiol. 13:814448. doi: [10.3389/fmicb.2022.814448] http://hdl.handle.net/10481/74844 10.3389/fmicb.2022.814448 eng http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess Atribución 3.0 España Frontiers