Physiological Doses of Hydroxytyrosol Modulate Gene Expression in Skeletal Muscle of Exercised Rats
Metadatos
Afficher la notice complèteAuteur
Casuso Pérez, Rafael Antonio; Al Fazazi, Saad; Plaza Díaz, Julio; Ruiz Ojeda, Francisco Javier; Rueda Robles, Ascensión; Aragón Vela, Jerónimo; Rodríguez Huertas, Jesús FranciscoEditorial
MDPI
Materia
Polyphenols Mitochondria Glycolysis Gene expression
Date
2021-12-12Referencia bibliográfica
Casuso, R.A... [et al.]. Physiological Doses of Hydroxytyrosol Modulate Gene Expression in Skeletal Muscle of Exercised Rats. Life 2021, 11, 1393. [https://doi.org/10.3390/life11121393]
Patrocinador
3650 CTS-454Résumé
We tested whether physiological doses of hydroxytyrosol (HT) may alter the mRNA transcription
of key metabolic genes in exercised skeletal muscle. Two groups of exercise-trained Wistar
rats, HTlow and HTmid, were supplemented with 0.31 and 4.61 mg/kg/d of HT, respectively, for
10 weeks. Another two groups of rats were not supplemented with HT; one remained sedentary
and the other one was exercised. After the experimental period, the soleus muscle was removed
for qRT-PCR and western blot analysis. The consumption of 4.61 mg/kg/d of HT during exercise
increased the mRNA expression of important metabolic proteins. Specifically, 4.61 mg/kg/d of HT
may upregulate long-chain fatty acid oxidation, lactate, and glucose oxidation as well as mitochondrial
Krebs cycle in trained skeletal muscle. However, a 4.61 mg/kg/d of HT may alter protein
translation, as in spite of the increment showed by CD36 and GLUT4 at the mRNA level this was not
translated to higher protein content.