Determination of the Main Ergot Alkaloids and Their Epimers in Oat-Based Functional Foods by Ultra-High Performance Liquid Chromatography Tandem Mass Spectrometry
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Carbonell Rozas, Laura; Gámiz Gracia, Laura; Lara Vargas, Francisco Jesús; García Campaña, Ana MaríaEditorial
MDPI
Materia
Ergot alkaloids Oats Food supplements QuEChERS UHPLC-MS/MS
Date
2021Referencia bibliográfica
Carbonell-Rozas, L.; Gámiz-Gracia, L.; Lara, F.J.; García-Campaña, A.M. Determination of the Main Ergot Alkaloids and Their Epimers in Oat-Based Functional Foods by Ultra-High Performance Liquid Chromatography Tandem Mass Spectrometry. Molecules 2021, 26, 3717. https://doi.org/10.3390/ molecules26123717
Sponsorship
Spanish Ministry of Science, Innovation, and Universities (project reference: RTI2018-097043-B-I00)Abstract
An ultra-high performance liquid chromatography coupled to tandem mass spectrometry method is proposed for the determination of the major ergot alkaloids (ergometrine, ergosine,
ergotamine, ergocornine, ergokryptine, ergocristine) and their epimers (ergometrinine, ergosinine,
ergotaminine, ergocorninine, ergokryptinine, and ergocristinine) in oat-based foods and food supplements. A modified QuEChERS (quick, easy, cheap, effective, rugged, and safe) procedure was applied
as sample treatment, reducing the consumption of organic solvent and increasing sensitivity. This
method involved an extraction with acetonitrile and ammonium carbonate (85:15, v/v) and a clean-up
step based on dispersive solid-phase extraction, employing a mixture of C18/Z-Sep+ as sorbents.
Procedural calibration curves were established and limits of quantification were below 3.2 µg/kg for
the studied compounds. Repeatability and intermediate precision (expressed as RSD%) were lower
than 6.3% and 15%, respectively, with recoveries ranging between 89.7% and 109%. The method was
applied to oat-based products (bran, flakes, flour, grass, hydroalcoholic extracts, juices, and tablets),
finding a positive sample of oat bran contaminated with ergometrine, ergosine, ergometrinine, and
ergosinine (total content of 10.7 µg/kg).