@misc{10481/69562,
year = {2021},
url = {http://hdl.handle.net/10481/69562},
abstract = {An ultra-high performance liquid chromatography coupled to tandem mass spectrometry method is proposed for the determination of the major ergot alkaloids (ergometrine, ergosine,
ergotamine, ergocornine, ergokryptine, ergocristine) and their epimers (ergometrinine, ergosinine,
ergotaminine, ergocorninine, ergokryptinine, and ergocristinine) in oat-based foods and food supplements. A modified QuEChERS (quick, easy, cheap, effective, rugged, and safe) procedure was applied
as sample treatment, reducing the consumption of organic solvent and increasing sensitivity. This
method involved an extraction with acetonitrile and ammonium carbonate (85:15, v/v) and a clean-up
step based on dispersive solid-phase extraction, employing a mixture of C18/Z-Sep+ as sorbents.
Procedural calibration curves were established and limits of quantification were below 3.2 µg/kg for
the studied compounds. Repeatability and intermediate precision (expressed as RSD%) were lower
than 6.3% and 15%, respectively, with recoveries ranging between 89.7% and 109%. The method was
applied to oat-based products (bran, flakes, flour, grass, hydroalcoholic extracts, juices, and tablets),
finding a positive sample of oat bran contaminated with ergometrine, ergosine, ergometrinine, and
ergosinine (total content of 10.7 µg/kg).},
organization = {Spanish Ministry of Science, Innovation, and Universities
(project reference: RTI2018-097043-B-I00)},
publisher = {MDPI},
keywords = {Ergot alkaloids},
keywords = {Oats},
keywords = {Food supplements},
keywords = {QuEChERS},
keywords = {UHPLC-MS/MS},
title = {Determination of the Main Ergot Alkaloids and Their Epimers in Oat-Based Functional Foods by Ultra-High Performance Liquid Chromatography Tandem Mass Spectrometry},
doi = {10.3390/molecules26123717},
author = {Carbonell Rozas, Laura and Gámiz Gracia, Laura and Lara Vargas, Francisco Jesús and García Campaña, Ana María},
}