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dc.contributor.authorCarbonell Rozas, Laura
dc.contributor.authorGámiz Gracia, Laura 
dc.contributor.authorLara Vargas, Francisco Jesús 
dc.contributor.authorGarcía Campaña, Ana María 
dc.date.accessioned2021-07-06T11:50:37Z
dc.date.available2021-07-06T11:50:37Z
dc.date.issued2021
dc.identifier.citationCarbonell-Rozas, L.; Gámiz-Gracia, L.; Lara, F.J.; García-Campaña, A.M. Determination of the Main Ergot Alkaloids and Their Epimers in Oat-Based Functional Foods by Ultra-High Performance Liquid Chromatography Tandem Mass Spectrometry. Molecules 2021, 26, 3717. https://doi.org/10.3390/ molecules26123717es_ES
dc.identifier.urihttp://hdl.handle.net/10481/69562
dc.description.abstractAn ultra-high performance liquid chromatography coupled to tandem mass spectrometry method is proposed for the determination of the major ergot alkaloids (ergometrine, ergosine, ergotamine, ergocornine, ergokryptine, ergocristine) and their epimers (ergometrinine, ergosinine, ergotaminine, ergocorninine, ergokryptinine, and ergocristinine) in oat-based foods and food supplements. A modified QuEChERS (quick, easy, cheap, effective, rugged, and safe) procedure was applied as sample treatment, reducing the consumption of organic solvent and increasing sensitivity. This method involved an extraction with acetonitrile and ammonium carbonate (85:15, v/v) and a clean-up step based on dispersive solid-phase extraction, employing a mixture of C18/Z-Sep+ as sorbents. Procedural calibration curves were established and limits of quantification were below 3.2 µg/kg for the studied compounds. Repeatability and intermediate precision (expressed as RSD%) were lower than 6.3% and 15%, respectively, with recoveries ranging between 89.7% and 109%. The method was applied to oat-based products (bran, flakes, flour, grass, hydroalcoholic extracts, juices, and tablets), finding a positive sample of oat bran contaminated with ergometrine, ergosine, ergometrinine, and ergosinine (total content of 10.7 µg/kg).es_ES
dc.description.sponsorshipSpanish Ministry of Science, Innovation, and Universities (project reference: RTI2018-097043-B-I00)es_ES
dc.language.isoenges_ES
dc.publisherMDPIes_ES
dc.rightsAtribución 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es/*
dc.subjectErgot alkaloidses_ES
dc.subjectOats es_ES
dc.subjectFood supplementses_ES
dc.subjectQuEChERSes_ES
dc.subjectUHPLC-MS/MSes_ES
dc.titleDetermination of the Main Ergot Alkaloids and Their Epimers in Oat-Based Functional Foods by Ultra-High Performance Liquid Chromatography Tandem Mass Spectrometryes_ES
dc.typejournal articlees_ES
dc.rights.accessRightsopen accesses_ES
dc.identifier.doi10.3390/molecules26123717


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