Generation and Evaluation of Novel Biomaterials Based on Decellularized Sturgeon Cartilage for Use in Tissue Engineering
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AuthorOrtiz Arrabal, Olimpia; Carmona Martos, Ramón; García García, Óscar Darío; Chato Astrain, Jesús; Sánchez Porras, David; Domezaín Fau, Alberto; Oruezabal, Roke Iñaki; Carriel Araya, Víctor; Campos Muñoz, Antonio Jesús
Ortiz-Arrabal, O.; Carmona, R.; García-García, Ó.D.; Chato-Astrain, J.; Sánchez-Porras, D.; Domezain, A.; Oruezabal, R.I.; Carriel, V.; Campos, A.; Alaminos, M. Generation and Evaluation of Novel Biomaterials Based on Decellularized Sturgeon Cartilage for Use in Tissue Engineering. Biomedicines 2021, 9, 775. https://doi. org/10.3390/biomedicines9070775
SponsorshipSpanish Plan Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica (I+D+i) of the Spanish Ministry of Economy and Competitiveness (Instituto de Salud Carlos III), Grant FIS PI20/0317; FEDER funds (European Union); Consejería de Salud y Familias, Junta de Andalucía, Spain - PI-0257-2017
Because cartilage has limited regenerative capability, a fully efficient advanced therapy medicinal product is needed to treat severe cartilage damage. We evaluated a novel biomaterial obtained by decellularizing sturgeon chondral endoskeleton tissue for use in cartilage tissue engineering. In silico analysis suggested high homology between human and sturgeon collagen proteins, and ultra-performance liquid chromatography confirmed that both types of cartilage consisted mainly of the same amino acids. Decellularized sturgeon cartilage was recellularized with human chondrocytes and four types of human mesenchymal stem cells (MSC) and their suitability for generating a cartilage substitute was assessed ex vivo and in vivo. The results supported the biocompatibility of the novel scaffold, as well as its ability to sustain cell adhesion, proliferation and differentiation. In vivo assays showed that the MSC cells in grafted cartilage disks were biosynthetically active and able to remodel the extracellular matrix of cartilage substitutes, with the production of type II collagen and other relevant components, especially when adipose tissue MSC were used. In addition, these cartilage substitutes triggered a pro-regenerative reaction mediated by CD206- positive M2 macrophages. These preliminary results warrant further research to characterize in greater detail the potential clinical translation of these novel cartilage substitutes.