@misc{10481/69552,
year = {2021},
url = {http://hdl.handle.net/10481/69552},
abstract = {Because cartilage has limited regenerative capability, a fully efficient advanced therapy
medicinal product is needed to treat severe cartilage damage. We evaluated a novel biomaterial
obtained by decellularizing sturgeon chondral endoskeleton tissue for use in cartilage tissue engineering. In silico analysis suggested high homology between human and sturgeon collagen proteins, and ultra-performance liquid chromatography confirmed that both types of cartilage consisted mainly of the same amino acids. Decellularized sturgeon cartilage was recellularized with
human chondrocytes and four types of human mesenchymal stem cells (MSC) and their suitability
for generating a cartilage substitute was assessed ex vivo and in vivo. The results supported the
biocompatibility of the novel scaffold, as well as its ability to sustain cell adhesion, proliferation and
differentiation. In vivo assays showed that the MSC cells in grafted cartilage disks were biosynthetically active and able to remodel the extracellular matrix of cartilage substitutes, with the production
of type II collagen and other relevant components, especially when adipose tissue MSC were used.
In addition, these cartilage substitutes triggered a pro-regenerative reaction mediated by CD206-
positive M2 macrophages. These preliminary results warrant further research to characterize in
greater detail the potential clinical translation of these novel cartilage substitutes.},
organization = {Spanish Plan Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica (I+D+i) of the Spanish Ministry of Economy and Competitiveness
(Instituto de Salud Carlos III), Grant FIS PI20/0317},
organization = {FEDER funds (European
Union)},
organization = {Consejería de Salud y Familias, Junta de Andalucía, Spain - PI-0257-2017},
publisher = {MDPI},
keywords = {Cartilage},
keywords = {Tissue engineering},
keywords = {Sturgeon},
keywords = {Decellularization},
title = {Generation and Evaluation of Novel Biomaterials Based on Decellularized Sturgeon Cartilage for Use in Tissue Engineering},
doi = {10.3390/biomedicines9070775},
author = {Ortiz Arrabal, Olimpia and Carmona Martos, Ramón and García García, Óscar Darío and Chato Astrain, Jesús and Sánchez Porras, David and Domezaín Fau, Alberto and Oruezabal, Roke Iñaki and Carriel Araya, Víctor and Campos Muñoz, Antonio Jesús},
}