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dc.contributor.authorOrtiz Arrabal, Olimpia
dc.contributor.authorCarmona Martos, Ramón 
dc.contributor.authorGarcía García, Óscar Darío
dc.contributor.authorChato Astrain, Jesús 
dc.contributor.authorSánchez Porras, David 
dc.contributor.authorDomezaín Fau, Alberto
dc.contributor.authorOruezabal, Roke Iñaki
dc.contributor.authorCarriel Araya, Víctor 
dc.contributor.authorCampos Muñoz, Antonio Jesús
dc.identifier.citationOrtiz-Arrabal, O.; Carmona, R.; García-García, Ó.D.; Chato-Astrain, J.; Sánchez-Porras, D.; Domezain, A.; Oruezabal, R.I.; Carriel, V.; Campos, A.; Alaminos, M. Generation and Evaluation of Novel Biomaterials Based on Decellularized Sturgeon Cartilage for Use in Tissue Engineering. Biomedicines 2021, 9, 775. https://doi. org/10.3390/biomedicines9070775es_ES
dc.description.abstractBecause cartilage has limited regenerative capability, a fully efficient advanced therapy medicinal product is needed to treat severe cartilage damage. We evaluated a novel biomaterial obtained by decellularizing sturgeon chondral endoskeleton tissue for use in cartilage tissue engineering. In silico analysis suggested high homology between human and sturgeon collagen proteins, and ultra-performance liquid chromatography confirmed that both types of cartilage consisted mainly of the same amino acids. Decellularized sturgeon cartilage was recellularized with human chondrocytes and four types of human mesenchymal stem cells (MSC) and their suitability for generating a cartilage substitute was assessed ex vivo and in vivo. The results supported the biocompatibility of the novel scaffold, as well as its ability to sustain cell adhesion, proliferation and differentiation. In vivo assays showed that the MSC cells in grafted cartilage disks were biosynthetically active and able to remodel the extracellular matrix of cartilage substitutes, with the production of type II collagen and other relevant components, especially when adipose tissue MSC were used. In addition, these cartilage substitutes triggered a pro-regenerative reaction mediated by CD206- positive M2 macrophages. These preliminary results warrant further research to characterize in greater detail the potential clinical translation of these novel cartilage substitutes.es_ES
dc.description.sponsorshipSpanish Plan Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica (I+D+i) of the Spanish Ministry of Economy and Competitiveness (Instituto de Salud Carlos III), Grant FIS PI20/0317es_ES
dc.description.sponsorshipFEDER funds (European Union)es_ES
dc.description.sponsorshipConsejería de Salud y Familias, Junta de Andalucía, Spain - PI-0257-2017es_ES
dc.rightsAtribución 3.0 España*
dc.subjectCartilage es_ES
dc.subjectTissue engineeringes_ES
dc.titleGeneration and Evaluation of Novel Biomaterials Based on Decellularized Sturgeon Cartilage for Use in Tissue Engineeringes_ES

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Atribución 3.0 España
Except where otherwise noted, this item's license is described as Atribución 3.0 España