Enhanced Stability Against Radiation Damage of Lysozyme Crys-tals Grown in Fmoc-CF Hydrogels
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Cryst. Growth Des. 2019, 19, 4229−4233
SponsorshipProjects BIO2016-74875-P (JAG) and FIS2017-85954-R (LAC) (Ministerio de Economía, Industria y Competitivid-ad, MINECO, and Agencia Estatal de Investigación, AEI, Spain, co-funded by Fondo Europeo de Desarrollo Region-al, FEDER, European Union) and by Junta de Andalucía (Spain) projects P12-FQM-2721 and P12-FQM-790.
Lysozyme crystals grown in Fmoc-CF (Cys-Phe) hydro-gels, unlike those grown in agarose, give rise to compo-site crystals that have an enhanced resistance against degradation caused by an intense exposure to X-ray irradiation. Fmoc-CF dipeptide shows a clear protection of the most sensitive groups (disulfide bonds and methi-onines) of the protein. The protection mediated by cys-teine is exerted only in its gel state since cysteine in solution has an adverse effect. Probably the reactive thiols groups of cysteine being locked within the rigid peptide fibers minimize cross-reactions with the proteins favoring the formation of protein crystals. Once located inside the protein crystal the long peptide fibers are able to protect the protein against radiation damage.