A New HPLC-MS Method for Measuring Maslinic Acid and Oleanolic Acid in HT29 and HepG2 Human Cancer Cells
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AuthorPeragón, Juan; Rufino Palomares, Eva; Muñoz Espada, Irene; Reyes Zurita, Fernando Jesús; Lupiáñez Cara, José Antonio
Maslinic acidOleanolic acidHPLC-MSHT29 cells
Peragón, J., Rufino-Palomares, E., Muñoz-Espada, I., Reyes-Zurita, F., & Lupiáñez, J. (2015). A new HPLC-MS method for measuring maslinic acid and oleanolic acid in HT29 and HepG2 human cancer cells. International journal of molecular sciences, 16(9), 21681-21694.
SponsorshipThis work was supported by research grant BIO-157 and BIO-341 of the Plan Andaluz de Investigación, Junta de Andalucía, Spain.
Maslinic acid (MA) and oleanolic acid (OA), the main triterpenic acids present in olive, have important properties for health and disease prevention. MA selectively inhibits cell proliferation of the HT29 human colon-cancer cell line by inducing selective apoptosis. For measuring the MA and OA concentration inside the cell and in the culture medium, a new HPLC-MS procedure has been developed. With this method, a determination of the amount of MA and OA incorporated into HT29 and HepG2 human cancer-cell lines incubated with different concentrations of MA corresponding to 50% growth inhibitory concentration (IC50), IC50/2, IC50/4, and IC50/8 has been made. The results demonstrate that this method is appropriate for determining the MA and OA concentration in different types of cultured cells and reveals the specific dynamics of entry of MA into HT29 and HepG2 cells.