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dc.contributor.authorPeragón, Juan
dc.contributor.authorRufino Palomares, Eva 
dc.contributor.authorMuñoz Espada, Irene
dc.contributor.authorReyes Zurita, Fernando Jesús 
dc.contributor.authorLupiáñez Cara, José Antonio
dc.identifier.citationPeragón, J., Rufino-Palomares, E., Muñoz-Espada, I., Reyes-Zurita, F., & Lupiáñez, J. (2015). A new HPLC-MS method for measuring maslinic acid and oleanolic acid in HT29 and HepG2 human cancer cells. International journal of molecular sciences, 16(9), 21681-21694.es_ES
dc.description.abstractMaslinic acid (MA) and oleanolic acid (OA), the main triterpenic acids present in olive, have important properties for health and disease prevention. MA selectively inhibits cell proliferation of the HT29 human colon-cancer cell line by inducing selective apoptosis. For measuring the MA and OA concentration inside the cell and in the culture medium, a new HPLC-MS procedure has been developed. With this method, a determination of the amount of MA and OA incorporated into HT29 and HepG2 human cancer-cell lines incubated with different concentrations of MA corresponding to 50% growth inhibitory concentration (IC50), IC50/2, IC50/4, and IC50/8 has been made. The results demonstrate that this method is appropriate for determining the MA and OA concentration in different types of cultured cells and reveals the specific dynamics of entry of MA into HT29 and HepG2 cells.es_ES
dc.description.sponsorshipThis work was supported by research grant BIO-157 and BIO-341 of the Plan Andaluz de Investigación, Junta de Andalucía, Spain.es_ES
dc.rightsAtribución 3.0 España*
dc.subjectMaslinic acides_ES
dc.subjectOleanolic acides_ES
dc.subjectHT29 cellses_ES
dc.titleA New HPLC-MS Method for Measuring Maslinic Acid and Oleanolic Acid in HT29 and HepG2 Human Cancer Cellses_ES
dc.accrual.periodicityCreative Commons Attribution-NonCommercial-NoDerivs 3.0 Licensees_ES

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Atribución 3.0 España
Except where otherwise noted, this item's license is described as Atribución 3.0 España