A New HPLC-MS Method for Measuring Maslinic Acid and Oleanolic Acid in HT29 and HepG2 Human Cancer Cells
Metadatos
Afficher la notice complèteAuteur
Peragón, Juan; Rufino Palomares, Eva; Muñoz Espada, Irene; Reyes Zurita, Fernando Jesús; Lupiáñez Cara, José AntonioEditorial
MDPI
Materia
Maslinic acid Oleanolic acid HPLC-MS HT29 cells
Date
2015-09-09Referencia bibliográfica
Peragón, J., Rufino-Palomares, E., Muñoz-Espada, I., Reyes-Zurita, F., & Lupiáñez, J. (2015). A new HPLC-MS method for measuring maslinic acid and oleanolic acid in HT29 and HepG2 human cancer cells. International journal of molecular sciences, 16(9), 21681-21694.
Patrocinador
This work was supported by research grant BIO-157 and BIO-341 of the Plan Andaluz de Investigación, Junta de Andalucía, Spain.Résumé
Maslinic acid (MA) and oleanolic acid (OA), the main triterpenic acids present in
olive, have important properties for health and disease prevention. MA selectively inhibits
cell proliferation of the HT29 human colon-cancer cell line by inducing selective apoptosis.
For measuring the MA and OA concentration inside the cell and in the culture medium, a
new HPLC-MS procedure has been developed. With this method, a determination of the
amount of MA and OA incorporated into HT29 and HepG2 human cancer-cell lines
incubated with different concentrations of MA corresponding to 50% growth inhibitory
concentration (IC50), IC50/2, IC50/4, and IC50/8 has been made. The results demonstrate that
this method is appropriate for determining the MA and OA concentration in different types
of cultured cells and reveals the specific dynamics of entry of MA into HT29 and HepG2 cells.