A New HPLC-MS Method for Measuring Maslinic Acid and Oleanolic Acid in HT29 and HepG2 Human Cancer Cells Peragón, Juan Rufino Palomares, Eva Muñoz Espada, Irene Reyes Zurita, Fernando Jesús Lupiáñez Cara, José Antonio Maslinic acid Oleanolic acid HPLC-MS HT29 cells Maslinic acid (MA) and oleanolic acid (OA), the main triterpenic acids present in olive, have important properties for health and disease prevention. MA selectively inhibits cell proliferation of the HT29 human colon-cancer cell line by inducing selective apoptosis. For measuring the MA and OA concentration inside the cell and in the culture medium, a new HPLC-MS procedure has been developed. With this method, a determination of the amount of MA and OA incorporated into HT29 and HepG2 human cancer-cell lines incubated with different concentrations of MA corresponding to 50% growth inhibitory concentration (IC50), IC50/2, IC50/4, and IC50/8 has been made. The results demonstrate that this method is appropriate for determining the MA and OA concentration in different types of cultured cells and reveals the specific dynamics of entry of MA into HT29 and HepG2 cells. 2019-12-03T10:13:39Z 2019-12-03T10:13:39Z 2015-09-09 info:eu-repo/semantics/article Peragón, J., Rufino-Palomares, E., Muñoz-Espada, I., Reyes-Zurita, F., & Lupiáñez, J. (2015). A new HPLC-MS method for measuring maslinic acid and oleanolic acid in HT29 and HepG2 human cancer cells. International journal of molecular sciences, 16(9), 21681-21694. http://hdl.handle.net/10481/58155 10.3390/ijms160921681 eng http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess Atribución 3.0 España MDPI