Alkyl sulfonyl derivatized PAMAM-G2 dendrimers as nonviral gene delivery vectors with improved transfection efficiencies
Metadatos
Mostrar el registro completo del ítemAutor
Morales Sanfrutos, Julia; Megía Fernández, Alicia; Hernández Mateo, Fernando; Girón González, María Dolores; Salto González, Rafael; Santoyo González, FranciscoEditorial
The Royal Society of Chemistry
Materia
PAMAM Dendrimers Gene transfection
Fecha
2011Referencia bibliográfica
Org. Biomol. Chem., 2011, 9, 851–864
Patrocinador
Direccion General de Investigacion Cientıfica y Tecnica (DGICYT) (CTQ2008-01754); Junta de Andalucıa (P07-FQM-02899); Fundacion Marcelino BotınResumen
Amphiphilic dendrimer-based gene delivery vectors bearing peripheral alkyl sulfonyl hydrophobic tails
were constructed using low-generation PAMAM-G2 as the core and functionalized by means of the
aza-Michael type addition of its primary amino groups to vinylsulfone derivatives as an efficient tool
for surface engineering. While the unmodified PAMAM-G2 was unable to efficiently transfect
eukaryotic cells, functionalized PAMAM-G2 dendrimers were able to bind DNA at low N/P ratios,
protect DNA from digestion with DNase I and showed high transfection efficiencies and low
cytotoxicity. Dendrimers with a C18 alkyl chain produced transfection efficiencies up to 3.1 fold higher
than LipofectAMINETM 2000 in CHO-k1 cells. The dendriplexes based in functionalized PAMAM-G2
also showed the ability to retain their transfection properties in the presence of serum and the ability to
transfect different eukaryotic cell lines such as Neuro-2A and RAW 264.7.