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Alkyl sulfonyl derivatized PAMAM-G2 dendrimers as nonviral gene delivery vectors with improved transfection efficiencies
dc.contributor.author | Morales Sanfrutos, Julia | |
dc.contributor.author | Megía Fernández, Alicia | |
dc.contributor.author | Hernández Mateo, Fernando | |
dc.contributor.author | Girón González, María Dolores | |
dc.contributor.author | Salto González, Rafael | |
dc.contributor.author | Santoyo González, Francisco | |
dc.date.accessioned | 2024-01-24T13:02:02Z | |
dc.date.available | 2024-01-24T13:02:02Z | |
dc.date.issued | 2011 | |
dc.identifier.citation | Org. Biomol. Chem., 2011, 9, 851–864 | es_ES |
dc.identifier.uri | https://hdl.handle.net/10481/87216 | |
dc.description | Financial Support was provided by Direccion General de Investigacion Cientıfica y Tecnica (DGICYT) (CTQ2008-01754) Junta de Andalucıa (P07-FQM-02899) and Fundacion Marcelino Botın. J. M.-S. thanks the University of Granada for a research contract (Programa Puente). A. M.-F. thanks the Spanish Ministerio de Educacion for a research fellowship (FPU). | es_ES |
dc.description.abstract | Amphiphilic dendrimer-based gene delivery vectors bearing peripheral alkyl sulfonyl hydrophobic tails were constructed using low-generation PAMAM-G2 as the core and functionalized by means of the aza-Michael type addition of its primary amino groups to vinylsulfone derivatives as an efficient tool for surface engineering. While the unmodified PAMAM-G2 was unable to efficiently transfect eukaryotic cells, functionalized PAMAM-G2 dendrimers were able to bind DNA at low N/P ratios, protect DNA from digestion with DNase I and showed high transfection efficiencies and low cytotoxicity. Dendrimers with a C18 alkyl chain produced transfection efficiencies up to 3.1 fold higher than LipofectAMINETM 2000 in CHO-k1 cells. The dendriplexes based in functionalized PAMAM-G2 also showed the ability to retain their transfection properties in the presence of serum and the ability to transfect different eukaryotic cell lines such as Neuro-2A and RAW 264.7. | es_ES |
dc.description.sponsorship | Direccion General de Investigacion Cientıfica y Tecnica (DGICYT) (CTQ2008-01754) | es_ES |
dc.description.sponsorship | Junta de Andalucıa (P07-FQM-02899) | es_ES |
dc.description.sponsorship | Fundacion Marcelino Botın | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | The Royal Society of Chemistry | es_ES |
dc.subject | PAMAM | es_ES |
dc.subject | Dendrimers | es_ES |
dc.subject | Gene transfection | es_ES |
dc.title | Alkyl sulfonyl derivatized PAMAM-G2 dendrimers as nonviral gene delivery vectors with improved transfection efficiencies | es_ES |
dc.type | journal article | es_ES |
dc.rights.accessRights | embargoed access | es_ES |
dc.identifier.doi | 10.1039/c0ob00355g | |
dc.type.hasVersion | VoR | es_ES |