@misc{10481/87216,
year = {2011},
url = {https://hdl.handle.net/10481/87216},
abstract = {Amphiphilic dendrimer-based gene delivery vectors bearing peripheral alkyl sulfonyl hydrophobic tails
were constructed using low-generation PAMAM-G2 as the core and functionalized by means of the
aza-Michael type addition of its primary amino groups to vinylsulfone derivatives as an efficient tool
for surface engineering. While the unmodified PAMAM-G2 was unable to efficiently transfect
eukaryotic cells, functionalized PAMAM-G2 dendrimers were able to bind DNA at low N/P ratios,
protect DNA from digestion with DNase I and showed high transfection efficiencies and low
cytotoxicity. Dendrimers with a C18 alkyl chain produced transfection efficiencies up to 3.1 fold higher
than LipofectAMINETM 2000 in CHO-k1 cells. The dendriplexes based in functionalized PAMAM-G2
also showed the ability to retain their transfection properties in the presence of serum and the ability to
transfect different eukaryotic cell lines such as Neuro-2A and RAW 264.7.},
organization = {Direccion General de Investigacion Cientıfica y Tecnica (DGICYT) (CTQ2008-01754)},
organization = {Junta de Andalucıa (P07-FQM-02899)},
organization = {Fundacion Marcelino Botın},
publisher = {The Royal Society of Chemistry},
keywords = {PAMAM},
keywords = {Dendrimers},
keywords = {Gene transfection},
title = {Alkyl sulfonyl derivatized PAMAM-G2 dendrimers as nonviral gene delivery vectors with improved transfection efficiencies},
doi = {10.1039/c0ob00355g},
author = {Morales Sanfrutos, Julia and Megía Fernández, Alicia and Hernández Mateo, Fernando and Girón González, María Dolores and Salto González, Rafael and Santoyo González, Francisco},
}