Optimization of the Extraction Conditions of Bioactive Compounds from Ocimum basilicum Leaves Using Ultrasound-Assisted Extraction via a Sonotrode
Metadatos
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MDPI
Materia
Sweet basil (Ocimum basilicum) Ultrasonic-assisted extraction Extraction via a sonotrode Box-Behnken design HPLC-ESI-TOF-MS
Fecha
2023-07-07Referencia bibliográfica
Aloisio, C.; Razola-Díaz, M.d.C.; Aznar-Ramos, M.J.; Longhi, M.R.; Andreatta, A.E.; Verardo, V. Optimization of the Extraction Conditions of Bioactive Compounds from Ocimum basilicum Leaves Using Ultrasound-Assisted Extraction via a Sonotrode. Molecules 2023, 28, 5286. [https://doi.org/10.3390/ molecules28135286]
Resumen
Sweet basil (Ocimum basilicum) leaves are rich in bioactive compounds that present therapeutic
benefits for human health. Ultrasonic-assisted extraction (UAE) is frequently used to obtain
phenolic compounds from plants/herbal sources. However, few works have developed multi-variable
studies to find the optimal conditions to extract the maximum amount of compounds, especially
when applied to UAE via a sonotrode. The purpose of this work was to perform a multi-variable
study by employing a Box–Behnken design to collect the highest active compound content from
Ocimum basilicum leaves. The efficacy of the design was endorsed by ANOVA. The studied parameters
for UAE via a sonotrode were the ethanol/water ratio, amplitude, and time. The analyzed responses
were the rosmarinic acid, the sum of phenolic acids, and the sum of phenolic compounds content. The
optimal conditions were found to be 50% ethanol/water, 50% amplitude, and 5 min. Twenty bioactive
compounds were identified by HPLC-ESI-TOF-MS when the extract was collected by applying the
optimal conditions. Ocimum basilicum may be appreciated as a valuable source of important bioactive
substances for pharmaceutical use