In vivo antitumor activity of Euphorbia lathyris ethanol extract in colon cancer models
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AuthorMesas Hernández, Cristina; Martínez Martínez, Rosario; Doello, Kevin; Ortiz Quesada, Raúl; López-Jurado Romero De La Cruz, María; Quiñonero Muñoz, Francisco José; Prados Salazar, José Carlos; Porres Foulquie, Jesús María; Melguizo Alonso, Consolación
Colon cancerEuphorbia lathyrisAOM/DSS modelOrthotopic xenograft modelOxidative stress detoxification enzymesMicrobiome
C. Mesas... [et al.]. In vivo antitumor activity of Euphorbia lathyris ethanol extract in colon cancer models, Biomedicine & Pharmacotherapy, Volume 149, 2022, 112883, ISSN 0753-3322, [https://doi.org/10.1016/j.biopha.2022.112883]
SponsorshipSpanish Government RTC-2017-6540-1 RTC2019-006870-1; Junta de Andalucia P18-TP-1420; European Commission AGR145 CTS-107; Andalusian Government
Euphorbia lathyris seeds have been used to treat various medical conditions. We previously reported that ethanolic extract from the defatted seed of Euphorbia lathyris (EE) (variety S3201) possesses a potent in vitro antitumor activity against colon cancer (CRC) cell lines. However, the effects of EE on CRC in vivo models and its possible preventive activity have not been elucidated. The aim of this study is to develop an in vivo study to corroborate its efficacy. For this purpose, two tumor induction models have been developed. In orthotopic xenograft model, it has been shown that EE reduces tumor size without hematological toxicity. The ethanolic extract induced an intense apoptosis in tumors mediated by caspase 3. Using the Azoxymethane/Dextran Sulfate Sodium model, a reduction of dysplastic polyps has been demonstrated, showing its preventive power. Furthermore, EE promoted the presence of an eubiotic microbiotal environment in the mucosa of the colon and induced an increase in antioxidant enzyme activity. This fact was accompanied by a modulation of cytokine expression that could be related to its protective mechanism. Therefore, although further experiments will be necessary to determine its applicability in the treatment of CRC, ES could be a new prevention strategy as well as treatment for this type of tumor, being a powerful candidate for future clinical trials.