Biological Effects of Maslinic Acid on Human Epithelial Cells Used in Tissue Engineering
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AuthorOrtiz Arrabal, Olimpia; Chato Astrain, Jesús; Crespo Ferrer, Pascual Vicente; Garzón Bello, Ingrid Johanna; Mesa García, María Dolores; Alaminos Mingorance, Miguel; Gómez Llorente, Carolina
Maslinic acidTissue engineeringEpithelial cellsCell cultureCell proliferation
Ortiz-Arrabal O... [et al.] (2022) Biological Effects of Maslinic Acid on Human Epithelial Cells Used in Tissue Engineering. Front. Bioeng. Biotechnol. 10:876734. doi: [10.3389/fbioe.2022.876734]
SponsorshipPE-0395-2019 Consejería de Salud y Familias, Junta de Andalucía, Spain; B-CTS-450-UGR20 (proyectos de I + D + i en el marco del Programa Operativo FEDER Andalucía 2014-2020, Universidad de Granada and Consejería de Transformación Económica, Industria, Conocimiento y Universidades); Spanish Plan Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica (I + D + i) of the Spanish Ministry of Science and Innovation through grants FIS PI20/0317, FIS PI21/ 0980 and ICI19-00024 (BIOCLEFT) from Instituto de Salud Carlos III, co-financed by the European Regional Development Fund
In the present work, we evaluated the potential of maslinic acid (MA) to improve currently available keratinocyte culture methods for use in skin tissue engineering. Results showed that MA can increase cell proliferation and WST-1 activity of human keratinocytes after 24, 48, and 72 h, especially at the concentration of 5 μg/ml, without affecting cell viability. This effect was associated to a significant increase of KI-67 protein expression and upregulation of several genes associated to cell proliferation (PCNA) and differentiation (cytokeratins, intercellular junctions and basement membrane related genes). When human keratinocytes were isolated from skin biopsies, we found that MA at the concentration of 5 μg/ml significantly increased the efficiency of the explant and the cell dissociation methods. These results revealed the positive effects of MA to optimize human keratinocyte culture protocols for use in skin tissue engineering.