Biological Effects of Maslinic Acid on Human Epithelial Cells Used in Tissue Engineering
Metadatos
Mostrar el registro completo del ítemAutor
Ortiz Arrabal, Olimpia; Chato Astrain, Jesús; Crespo Ferrer, Pascual Vicente; Garzón Bello, Ingrid Johanna; Mesa García, María Dolores; Alaminos Mingorance, Miguel; Gómez Llorente, CarolinaEditorial
Frontiers
Materia
Maslinic acid Tissue engineering Epithelial cells Cell culture Cell proliferation
Fecha
2022-04-27Referencia bibliográfica
Ortiz-Arrabal O... [et al.] (2022) Biological Effects of Maslinic Acid on Human Epithelial Cells Used in Tissue Engineering. Front. Bioeng. Biotechnol. 10:876734. doi: [10.3389/fbioe.2022.876734]
Patrocinador
PE-0395-2019 Consejería de Salud y Familias, Junta de Andalucía, Spain; B-CTS-450-UGR20 (proyectos de I + D + i en el marco del Programa Operativo FEDER Andalucía 2014-2020, Universidad de Granada and Consejería de Transformación Económica, Industria, Conocimiento y Universidades); Spanish Plan Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica (I + D + i) of the Spanish Ministry of Science and Innovation through grants FIS PI20/0317, FIS PI21/ 0980 and ICI19-00024 (BIOCLEFT) from Instituto de Salud Carlos III, co-financed by the European Regional Development FundResumen
In the present work, we evaluated the potential of maslinic acid (MA) to improve currently
available keratinocyte culture methods for use in skin tissue engineering. Results showed
that MA can increase cell proliferation and WST-1 activity of human keratinocytes after 24,
48, and 72 h, especially at the concentration of 5 μg/ml, without affecting cell viability. This
effect was associated to a significant increase of KI-67 protein expression and
upregulation of several genes associated to cell proliferation (PCNA) and differentiation
(cytokeratins, intercellular junctions and basement membrane related genes). When
human keratinocytes were isolated from skin biopsies, we found that MA at the
concentration of 5 μg/ml significantly increased the efficiency of the explant and the cell
dissociation methods. These results revealed the positive effects of MA to optimize human
keratinocyte culture protocols for use in skin tissue engineering.