Biological Effects of Maslinic Acid on Human Epithelial Cells Used in Tissue Engineering Ortiz Arrabal, Olimpia Chato Astrain, Jesús Crespo Ferrer, Pascual Vicente Garzón Bello, Ingrid Johanna Mesa García, María Dolores Alaminos Mingorance, Miguel Gómez Llorente, Carolina Maslinic acid Tissue engineering Epithelial cells Cell culture Cell proliferation In the present work, we evaluated the potential of maslinic acid (MA) to improve currently available keratinocyte culture methods for use in skin tissue engineering. Results showed that MA can increase cell proliferation and WST-1 activity of human keratinocytes after 24, 48, and 72 h, especially at the concentration of 5 μg/ml, without affecting cell viability. This effect was associated to a significant increase of KI-67 protein expression and upregulation of several genes associated to cell proliferation (PCNA) and differentiation (cytokeratins, intercellular junctions and basement membrane related genes). When human keratinocytes were isolated from skin biopsies, we found that MA at the concentration of 5 μg/ml significantly increased the efficiency of the explant and the cell dissociation methods. These results revealed the positive effects of MA to optimize human keratinocyte culture protocols for use in skin tissue engineering. 2022-06-22T10:07:09Z 2022-06-22T10:07:09Z 2022-04-27 info:eu-repo/semantics/article Ortiz-Arrabal O... [et al.] (2022) Biological Effects of Maslinic Acid on Human Epithelial Cells Used in Tissue Engineering. Front. Bioeng. Biotechnol. 10:876734. doi: [10.3389/fbioe.2022.876734] http://hdl.handle.net/10481/75601 10.3389/fbioe.2022.876734 eng http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess Atribución 3.0 España Frontiers