@misc{10481/75601,
year = {2022},
month = {4},
url = {http://hdl.handle.net/10481/75601},
abstract = {In the present work, we evaluated the potential of maslinic acid (MA) to improve currently
available keratinocyte culture methods for use in skin tissue engineering. Results showed
that MA can increase cell proliferation and WST-1 activity of human keratinocytes after 24,
48, and 72 h, especially at the concentration of 5 μg/ml, without affecting cell viability. This
effect was associated to a significant increase of KI-67 protein expression and
upregulation of several genes associated to cell proliferation (PCNA) and differentiation
(cytokeratins, intercellular junctions and basement membrane related genes). When
human keratinocytes were isolated from skin biopsies, we found that MA at the
concentration of 5 μg/ml significantly increased the efficiency of the explant and the cell
dissociation methods. These results revealed the positive effects of MA to optimize human
keratinocyte culture protocols for use in skin tissue engineering.},
organization = {PE-0395-2019 Consejería de Salud y Familias, Junta de Andalucía, Spain},
organization = {B-CTS-450-UGR20 (proyectos de I + D + i en el marco del Programa Operativo FEDER Andalucía 2014-2020, Universidad
de Granada and Consejería de Transformación Económica,
Industria, Conocimiento y Universidades)},
organization = {Spanish Plan Nacional de Investigación Científica, Desarrollo e
Innovación Tecnológica (I + D + i) of the Spanish Ministry of
Science and Innovation through grants FIS PI20/0317, FIS PI21/
0980 and ICI19-00024 (BIOCLEFT) from Instituto de Salud
Carlos III, co-financed by the European Regional Development
Fund},
publisher = {Frontiers},
keywords = {Maslinic acid},
keywords = {Tissue engineering},
keywords = {Epithelial cells},
keywords = {Cell culture},
keywords = {Cell proliferation},
title = {Biological Effects of Maslinic Acid on Human Epithelial Cells Used in Tissue Engineering},
doi = {10.3389/fbioe.2022.876734},
author = {Ortiz Arrabal, Olimpia and Chato Astrain, Jesús and Crespo Ferrer, Pascual Vicente and Garzón Bello, Ingrid Johanna and Mesa García, María Dolores and Alaminos Mingorance, Miguel and Gómez Llorente, Carolina},
}