Injectable Magnetic-Responsive Short-Peptide Supramolecular Hydrogels: Ex Vivo and In Vivo Evaluation
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AuthorMañas Torres, María del Carmen; Gila Vilchez, Cristina; Vázquez Pérez, Francisco Jesús; Blanco Elices, Cristina; Rodríguez, Ismael Ángel; Alaminos Mingorance, Miguel; Álvarez Cienfuegos Rodríguez, Luis; López López, Modesto Torcuato
American Chemical Society
PeptidesHybrid hydrogelsBiomaterialsMagnetic nanoparticlesSelf-assemblyTissue engineeringRegenerative medicine
ACS Appl. Mater. Interfaces 2021, 13, 49692−49704. [https://doi.org/10.1021/acsami.1c13972]
SponsorshipInstituto de Salud Carlos III FIS PI20/0317 ICI19/00024; European Commission; FSE "El FSE invierte en tu futuro", Spain; French National Research Agency (ANR) ANR-15-IDEX-01; Universidad de Granada/CBUA; FIS2017-85954-R MCIN/AEI/10.13039/501100011033/FEDER PE-0395-2019 PPJIB2020.07 PRE2018-083773 MCIN/AEI/10.13039/501100011033 FPU17/00491
The inclusion of magnetic nanoparticles (MNP) in a hydrogel matrix to produce magnetic hydrogels has broadened the scope of these materials in biomedical research. Embedded MNP offer the possibility to modulate the physical properties of the hydrogel remotely and on demand by applying an external magnetic field. Moreover, they enable permanent changes in the mechanical properties of the hydrogel, as well as alterations in the micro- and macroporosity of its threedimensional (3D) structure, with the associated potential to induce anisotropy. In this work, the behavior of biocompatible and biodegradable hydrogels made with Fmoc-diphenylalanine (Fmoc-FF) (Fmoc = fluorenylmethoxycarbonyl) and Fmoc−arginine−glycine− aspartic acid (Fmoc-RGD) short peptides to which MNP were incorporated was studied in detail with physicochemical, mechanical, and biological methods. The resulting hybrid hydrogels showed enhance mechanical properties and withstood injection without phase disruption. In mice, the hydrogels showed faster and improved self-healing properties compared to their nonmagnetic counterparts. Thanks to these superior physical properties and stability during culture, they can be used as 3D scaffolds for cell growth. Additionally, magnetic short-peptide hydrogels showed good biocompatibility and the absence of toxicity, which together with their enhanced mechanical stability and excellent injectability make them ideal biomaterials for in vivo biomedical applications with minimally invasive surgery. This study presents a new approach to improving the physical and mechanical properties of supramolecular hydrogels by incorporating MNP, which confer structural reinforcement and stability, remote actuation by magnetic fields, and better injectability. Our approach is a potential catalyst for expanding the biomedical applications of supramolecular short-peptide hydrogels.