Polymeric nanoparticles for endodontic therapy
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AuthorToledano Pérez, Manuel; Osorio Ruiz, María Estrella; Sánchez Aguilera, Fátima; Muñoz Soto, Esther; Toledano-Osorio, Manuel; López López, Modesto Torcuato; Medina Castillo, Antonio Luis; Carmona-Carmona, Álvaro; Osorio Ruiz, Raquel
ELSEVIER SCIENCE BV
Toledano M., Osorio E., Aguilera F.S., Muñoz-Soto E., Toledano-Osorio M., López-López M.T., Medina-Castillo A.L., Carrasco-Carmona Á., Osorio R. Polymeric nanoparticles for endodontic therapy (2020) Journal of the Mechanical Behavior of Biomedical Materials, 103.: 103606
SponsorshipThis work was supported by the Ministry of Economy and Competitiveness and European Regional Development Fund [MAT2017-85999P MINECO/AEI/FEDER/UE].
The effectiveness of novel polymeric nanoparticles (NPs) application in reducing dentin permeability and facilitating dentin remineralization after endodontic treatment was evaluated. The effect of undoped NPs, zinc, calcium and doxycycline-doped NPs (Zn-NPs, Ca-NPs and D-NPs, respectively) was tested in radicular dentin. A control group without NPs was included. Radicular dentin was assessed for fluid filtration. Dentin remineralization was analyzed by scanning and transmission electron microscopy, energy-dispersive analysis, AFM, Young´s modulus (Ei), Nano DMA, Raman, and X-Ray Diffraction analysis. Ca-NPs and Zn-NPs treated dentin exhibited the lowest microleakage with hermetically sealed dentinal tubules and a zinc-based salt generation onto dentin. Zn-NPs favored crystallinity and promoted the highest Ei and functional remineralization at the apical dentin, generating differences between the values of complex modulus among groups. Ca-NPs produced closure of tubules and porosities at the expense of a relative mineral amorphization, without creating zones of stress concentration. The highest sealing efficacy was obtained in Zn-NPs-treated samples, along with the highest values of Young´s modulus and dentin mineralization. These high values of Ei were obtained by closing voids, cracks, pores and tubules, and by strengthening the root dentin. When using undoped NPs or Ca-NPs, deposition of minerals occurred, but radicular dentin was not mechanically reinforced. Therefore, application of Zn-NPs in endodontically treated teeth previous to the canal filling is encouraged.