Polymeric nanoparticles for endodontic therapy Toledano Pérez, Manuel Osorio Ruiz, María Estrella Sánchez Aguilera, Fátima Muñoz Soto, Esther Toledano Osorio, Manuel López López, Modesto Torcuato Medina Castillo, Antonio Luis Carmona-Carmona, Álvaro Osorio Ruiz, Raquel endodontics mineralization NanoDMA nanoparticles Raman sealing ability The effectiveness of novel polymeric nanoparticles (NPs) application in reducing dentin permeability and facilitating dentin remineralization after endodontic treatment was evaluated. The effect of undoped NPs, zinc, calcium and doxycycline-doped NPs (Zn-NPs, Ca-NPs and D-NPs, respectively) was tested in radicular dentin. A control group without NPs was included. Radicular dentin was assessed for fluid filtration. Dentin remineralization was analyzed by scanning and transmission electron microscopy, energy-dispersive analysis, AFM, Young´s modulus (Ei), Nano DMA, Raman, and X-Ray Diffraction analysis. Ca-NPs and Zn-NPs treated dentin exhibited the lowest microleakage with hermetically sealed dentinal tubules and a zinc-based salt generation onto dentin. Zn-NPs favored crystallinity and promoted the highest Ei and functional remineralization at the apical dentin, generating differences between the values of complex modulus among groups. Ca-NPs produced closure of tubules and porosities at the expense of a relative mineral amorphization, without creating zones of stress concentration. The highest sealing efficacy was obtained in Zn-NPs-treated samples, along with the highest values of Young´s modulus and dentin mineralization. These high values of Ei were obtained by closing voids, cracks, pores and tubules, and by strengthening the root dentin. When using undoped NPs or Ca-NPs, deposition of minerals occurred, but radicular dentin was not mechanically reinforced. Therefore, application of Zn-NPs in endodontically treated teeth previous to the canal filling is encouraged. 2020-05-18T11:45:38Z 2020-05-18T11:45:38Z 2020 info:eu-repo/semantics/article Toledano M., Osorio E., Aguilera F.S., Muñoz-Soto E., Toledano-Osorio M., López-López M.T., Medina-Castillo A.L., Carrasco-Carmona Á., Osorio R. Polymeric nanoparticles for endodontic therapy (2020) Journal of the Mechanical Behavior of Biomedical Materials, 103.: 103606 http://hdl.handle.net/10481/62103 https://doi.org/10.1016/j.jmbbm.2019.10360 eng http://creativecommons.org/licenses/by-nc-nd/3.0/es/ info:eu-repo/semantics/openAccess Atribución-NoComercial-SinDerivadas 3.0 España ELSEVIER SCIENCE BV