Antihypertensive Effects of Virgin Olive Oil (Unfiltered) Low Molecular Weight Peptides with ACE Inhibitory Activity in Spontaneously Hypertensive Rats
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Alcaide Hidalgo, Juan María; Romero, Miguel; Duarte Pérez, Juan Manuel; López Huertas León, EduardoEditorial
MDPI
Materia
Unfiltered virgin olive oil Spontaneously hypertensive rats Peptides Hypertension ACE
Date
2020-01-20Referencia bibliográfica
Alcaide-Hidalgo, J. M., Romero, M., Duarte, J., & López-Huertas, E. (2020). Antihypertensive effects of virgin olive oil (unfiltered) low molecular weight peptides with ACE inhibitory activity in spontaneously hypertensive rats. Nutrients, 12(1), 271.
Sponsorship
This research was funded by the European Regional Development Funds under the agreement signed between MINECO and CSIC for the realization of the RECUPERA 2020 project.Abstract
The low molecular weight peptide composition of virgin olive oil (VOO) is mostly
unknown. We hypothesised that unfiltered VOO could possess low molecular weight peptides with
antihypertensive activity. We produced unfiltered VOO and obtained a water-soluble peptide extract
from it. The peptides were separated by size-exclusion using fast protein liquid chromatography,
and the low molecular weight fraction was analysed by nanoscale liquid chromatography-Orbitrap
coupled with tandem mass spectrometry and de novo sequencing. We selected 23 peptide sequences
containing between 6 and 9 amino acids and molecular masses ranging 698–1017 Da. Those peptides
were chemically synthesised and their angiotensin-converting enzyme (ACE) inhibitory activity was
studied in vitro. Seven peptides showed a strong activity, with half maximal inhibitory concentration
(IC50) <10 um. The antihypertensive effects of the four most active synthesised ACE inhibitor
peptides were studied in spontaneously hypertensive rats (SHR). Acute oral administration of
synthetic peptides RDGGYCC and CCGNAVPQ showed antihypertensive activity in SHR. We
conclude that unfiltered VOO naturally contains low molecular weight peptides with specific ACE
inhibitory activity and antihypertensive effects in SHR.