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dc.contributor.authorAlcaide Hidalgo, Juan María
dc.contributor.authorRomero, Miguel
dc.contributor.authorDuarte Pérez, Juan Manuel 
dc.contributor.authorLópez Huertas León, Eduardo
dc.date.accessioned2020-04-15T11:45:45Z
dc.date.available2020-04-15T11:45:45Z
dc.date.issued2020-01-20
dc.identifier.citationAlcaide-Hidalgo, J. M., Romero, M., Duarte, J., & López-Huertas, E. (2020). Antihypertensive effects of virgin olive oil (unfiltered) low molecular weight peptides with ACE inhibitory activity in spontaneously hypertensive rats. Nutrients, 12(1), 271.es_ES
dc.identifier.urihttp://hdl.handle.net/10481/61257
dc.description.abstractThe low molecular weight peptide composition of virgin olive oil (VOO) is mostly unknown. We hypothesised that unfiltered VOO could possess low molecular weight peptides with antihypertensive activity. We produced unfiltered VOO and obtained a water-soluble peptide extract from it. The peptides were separated by size-exclusion using fast protein liquid chromatography, and the low molecular weight fraction was analysed by nanoscale liquid chromatography-Orbitrap coupled with tandem mass spectrometry and de novo sequencing. We selected 23 peptide sequences containing between 6 and 9 amino acids and molecular masses ranging 698–1017 Da. Those peptides were chemically synthesised and their angiotensin-converting enzyme (ACE) inhibitory activity was studied in vitro. Seven peptides showed a strong activity, with half maximal inhibitory concentration (IC50) <10 um. The antihypertensive effects of the four most active synthesised ACE inhibitor peptides were studied in spontaneously hypertensive rats (SHR). Acute oral administration of synthetic peptides RDGGYCC and CCGNAVPQ showed antihypertensive activity in SHR. We conclude that unfiltered VOO naturally contains low molecular weight peptides with specific ACE inhibitory activity and antihypertensive effects in SHR.es_ES
dc.description.sponsorshipThis research was funded by the European Regional Development Funds under the agreement signed between MINECO and CSIC for the realization of the RECUPERA 2020 project.es_ES
dc.language.isoenges_ES
dc.publisherMDPIes_ES
dc.rightsAtribución 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es/*
dc.subjectUnfiltered virgin olive oiles_ES
dc.subjectSpontaneously hypertensive ratses_ES
dc.subjectPeptides es_ES
dc.subjectHypertension es_ES
dc.subjectACEes_ES
dc.titleAntihypertensive Effects of Virgin Olive Oil (Unfiltered) Low Molecular Weight Peptides with ACE Inhibitory Activity in Spontaneously Hypertensive Ratses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES
dc.identifier.doi10.3390/nu12010271


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Atribución 3.0 España
Except where otherwise noted, this item's license is described as Atribución 3.0 España