<rdf:RDF xmlns:rdf="http://www.openarchives.org/OAI/2.0/rdf/" xmlns:ow="http://www.ontoweb.org/ontology/1#" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:ds="http://dspace.org/ds/elements/1.1/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:doc="http://www.lyncode.com/xoai" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/rdf/ http://www.openarchives.org/OAI/2.0/rdf.xsd">
   <ow:Publication rdf:about="oai:digibug.ugr.es:10481/61257">
      <dc:title>Antihypertensive Effects of Virgin Olive Oil (Unfiltered) Low Molecular Weight Peptides with ACE Inhibitory Activity in Spontaneously Hypertensive Rats</dc:title>
      <dc:creator>Alcaide Hidalgo, Juan María</dc:creator>
      <dc:creator>Romero, Miguel</dc:creator>
      <dc:creator>Duarte Pérez, Juan Manuel</dc:creator>
      <dc:creator>López Huertas León, Eduardo</dc:creator>
      <dc:subject>Unfiltered virgin olive oil</dc:subject>
      <dc:subject>Spontaneously hypertensive rats</dc:subject>
      <dc:subject>Peptides</dc:subject>
      <dc:subject>Hypertension</dc:subject>
      <dc:subject>ACE</dc:subject>
      <dc:description>The low molecular weight peptide composition of virgin olive oil (VOO) is mostly&#xd;
unknown. We hypothesised that unfiltered VOO could possess low molecular weight peptides with&#xd;
antihypertensive activity. We produced unfiltered VOO and obtained a water-soluble peptide extract&#xd;
from it. The peptides were separated by size-exclusion using fast protein liquid chromatography,&#xd;
and the low molecular weight fraction was analysed by nanoscale liquid chromatography-Orbitrap&#xd;
coupled with tandem mass spectrometry and de novo sequencing. We selected 23 peptide sequences&#xd;
containing between 6 and 9 amino acids and molecular masses ranging 698–1017 Da. Those peptides&#xd;
were chemically synthesised and their angiotensin-converting enzyme (ACE) inhibitory activity was&#xd;
studied in vitro. Seven peptides showed a strong activity, with half maximal inhibitory concentration&#xd;
(IC50) &lt;10 um. The antihypertensive effects of the four most active synthesised ACE inhibitor&#xd;
peptides were studied in spontaneously hypertensive rats (SHR). Acute oral administration of&#xd;
synthetic peptides RDGGYCC and CCGNAVPQ showed antihypertensive activity in SHR. We&#xd;
conclude that unfiltered VOO naturally contains low molecular weight peptides with specific ACE&#xd;
inhibitory activity and antihypertensive effects in SHR.</dc:description>
      <dc:date>2020-04-15T11:45:45Z</dc:date>
      <dc:date>2020-04-15T11:45:45Z</dc:date>
      <dc:date>2020-01-20</dc:date>
      <dc:type>journal article</dc:type>
      <dc:identifier>Alcaide-Hidalgo, J. M., Romero, M., Duarte, J., &amp; López-Huertas, E. (2020). Antihypertensive effects of virgin olive oil (unfiltered) low molecular weight peptides with ACE inhibitory activity in spontaneously hypertensive rats. Nutrients, 12(1), 271.</dc:identifier>
      <dc:identifier>http://hdl.handle.net/10481/61257</dc:identifier>
      <dc:identifier>10.3390/nu12010271</dc:identifier>
      <dc:language>eng</dc:language>
      <dc:rights>http://creativecommons.org/licenses/by/3.0/es/</dc:rights>
      <dc:rights>open access</dc:rights>
      <dc:rights>Atribución 3.0 España</dc:rights>
      <dc:publisher>MDPI</dc:publisher>
   </ow:Publication>
</rdf:RDF>