Genomic analyses of two Alteromonas stellipolaris strains reveal traits with potential biotechnological applications
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AuthorTorres, Marta; Hong, Kar-Wai; Chong, Teik-Min; Reina, José Carlos; Chan, Kok-Gan; Dessaux; Dessaux, Yves; Llamas Company, Inmaculada
Torres, M., Hong, K. W., Chong, T. M., Reina, J. C., Chan, K. G., Dessaux, Y., & Llamas, I. (2019). Genomic analyses of two Alteromonas stellipolaris strains reveal traits with potential biotechnological applications. Scientific reports, 9(1), 1-14.
SponsorshipI.L. was supported by a Spanish Ministry of Economy, Industry and Competitiveness research project (AGL2015- 68806-R); Y.D. by an annual grant from the French National Scientific Research Center (CNRS); Y.D. and K.G.C. by the French Embassy in Malaysia and K.G.C. by the University of Malaysia (grants H-50001-A000027 and GA001-2016). The Institute for Integrative Biology of the Cell also received support from the LabEx Saclay Plant Sciences-SPS (ANR-10-LABX-0040-SPS). M.T. was supported by an FPU Fellowship (FPU13-0466) and the International Mobility Programme (EST15/00709) of the Spanish Ministry of Science and Education. K.W.H. and T.M.C. received grants from the French Embassy in Malaysia. J.C.R. was supported by an FPU Fellowship grant (FPU15-01717).
The Alteromonas stellipolaris strains PQQ-42 and PQQ-44, previously isolated from a fish hatchery, have been selected on the basis of their strong quorum quenching (QQ) activity, as well as their ability to reduce Vibrio-induced mortality on the coral Oculina patagonica. In this study, the genome sequences of both strains were determined and analyzed in order to identify the mechanism responsible for QQ activity. Both PQQ-42 and PQQ-44 were found to degrade a wide range of N-acylhomoserine lactone (AHL) QS signals, possibly due to the presence of an aac gene which encodes an AHL amidohydrolase. In addition, the different colony morphologies exhibited by the strains could be related to the differences observed in genes encoding cell wall biosynthesis and exopolysaccharide (EPS) production. The PQQ-42 strain produces more EPS (0.36 g l−1) than the PQQ-44 strain (0.15 g l−1), whose chemical compositions also differ. Remarkably, PQQ-44 EPS contains large amounts of fucose, a sugar used in high-value biotechnological applications. Furthermore, the genome of strain PQQ-42 contained a large nonribosomal peptide synthase (NRPS) cluster with a previously unknown genetic structure. The synthesis of enzymes and other bioactive compounds were also identified, indicating that PQQ-42 and PQQ-44 could have biotechnological applications.