Thread based microfluidic platform for urinary creatinine analysis
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AuthorArroyo, Manuel J.; Erenas Rodríguez, Miguel María; Orbe Payá, Ignacio De; Dobado Jiménez, José Antonio; Ballester, Pablo; Blondeau, Pascal; Salinas Castillo, Alfonso; Capitán Vallvey, Luis Fermín; Cantrell, Kevin
Optical deviceµTADCreatinine determinationDensity functional theoryCalixpyrrole derivativeSmartphone
Sensors & Actuators: B. Chemical 305 (2020) 127407
Sponsorship“Ministerio de Economía y Competitividad” under Project CTQ2016-78754-C2-1-R
Creatinine level in urine is a key factor to monitor kidney performance. The use of an alternative microfluidic platform based on cellulose substrates is an interesting option to integrate sample treatment, creatinine re- cognition by ionophore extraction chemistry and quantification by color measurement through consumer electronics imaging devices. The inclusion of ionophore extraction chemistry based on aryl-substituted calix pyrrole synthetic receptor on 8.7 mm long cotton thread permit the sample treatment, optical recognition of creatinine and their quantification by smartphone running app in unfiltered urine samples diluted 1:100 ratio. The device shows a short response time, 30 s, to creatinine over a wide dynamic range (from 1.6× 10 6 to 5 10–2 M) with precision between 2.9–4.3%. The low interference level of representative species in urine is studied and justified by density functional theory (DFT) calculations.