Real time monitoring of glucose in whole blood by smartphone

Erenas Rodríguez, Miguel María; Carrillo-Aguilera, Belén; Cantrell, Kevin; González-Chocano, Sara; Pérez de Vargas Sansalvador, Isabel María; Orbe Payá, Ignacio De; Capitán Vallvey, Luis Fermín

doi:10.1016/j.bios.2019.04.024

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Supplementary information (856.9Kb)

Identificadores

URI: http://hdl.handle.net/10481/55714

DOI: 10.1016/j.bios.2019.04.024

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Materia

Thread-paper microfluidic device

Glucose determination

Whole blood

Reaction rate

Smartphone

Date

2019-07

Referencia bibliográfica

Erenas, Miguel María [et al.] Real time monitoring of glucose in whole blood by smartphone. Biosensors and Bioelectronics 136 (2019) 47–52

Sponsorship

This study was supported by the CTQ2016-78754-C2-1-R project from the Spanish MINECO

Abstract

A combined thread-paper microfluidic device (μTPAD) is presented for the determination of glucose in blood. The device is designed to include all the analytical operations needed: red blood cell separation, conditioning, enzymatic recognition, and colorimetric transduction. The signal is captured with a smartphone or tablet working in video mode and processed by custom Android-based software in real-time. The automatic detection of the region of interest on the thread allows for the use of either initial rate or equilibrium signal as analytical parameters. The time needed for analysis is 12 s using initial rate, and 100 s using the equilibrium measurement with a LOD of 48 μM and 12 μM, respectively, and a precision around 7%. The μTPAD allows a rapid de- termination of glucose in real samples using only 3 μL of whole blood.