Real time monitoring of glucose in whole blood by smartphone Erenas Rodríguez, Miguel María Carrillo-Aguilera, Belén Cantrell, Kevin González-Chocano, Sara Pérez de Vargas Sansalvador, Isabel María Orbe Payá, Ignacio De Capitán Vallvey, Luis Fermín Thread-paper microfluidic device Glucose determination Whole blood Reaction rate Smartphone A combined thread-paper microfluidic device (μTPAD) is presented for the determination of glucose in blood. The device is designed to include all the analytical operations needed: red blood cell separation, conditioning, enzymatic recognition, and colorimetric transduction. The signal is captured with a smartphone or tablet working in video mode and processed by custom Android-based software in real-time. The automatic detection of the region of interest on the thread allows for the use of either initial rate or equilibrium signal as analytical parameters. The time needed for analysis is 12 s using initial rate, and 100 s using the equilibrium measurement with a LOD of 48 μM and 12 μM, respectively, and a precision around 7%. The μTPAD allows a rapid de- termination of glucose in real samples using only 3 μL of whole blood. 2019-05-14T06:21:00Z 2019-05-14T06:21:00Z 2019-07 info:eu-repo/semantics/article Erenas, Miguel María [et al.] Real time monitoring of glucose in whole blood by smartphone. Biosensors and Bioelectronics 136 (2019) 47–52 http://hdl.handle.net/10481/55714 10.1016/j.bios.2019.04.024 eng info:eu-repo/semantics/embargoedAccess