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dc.contributor.authorOlmo García, Lucía 
dc.contributor.authorKessler, Nikolas
dc.contributor.authorNeuweger, Heiko
dc.contributor.authorWendt, Karin
dc.contributor.authorOlmo-Peinado, José María
dc.contributor.authorFernández Gutiérrez, Alberto 
dc.contributor.authorBaessmann, Carsten
dc.contributor.authorCarrasco Pancorbo, Alegría 
dc.date.accessioned2019-04-08T12:29:30Z
dc.date.available2019-04-08T12:29:30Z
dc.date.issued2018-09-20
dc.identifier.citationOlmo García, L. [et al.]. Unravelling the Distribution of Secondary Metabolites in Olea europaea L.: Exhaustive Characterization of Eight Olive-Tree Derived Matrices by Complementary Platforms. Molecules 2018, 23, 2419; doi:10.3390/molecules23102419.es_ES
dc.identifier.issn1420-3049
dc.identifier.urihttp://hdl.handle.net/10481/55391
dc.description.abstractIn order to understand the distribution of the main secondary metabolites found in Olea europaea L., eight different samples (olive leaf, stem, seed, fruit skin and pulp, as well as virgin olive oil, olive oil obtained from stoned and dehydrated fruits and olive seed oil) coming from a Picudo cv. olive tree were analyzed. All the experimental conditions were selected so as to assure the maximum coverage of the metabolome of the samples under study within a single run. The use of LC and GC with high resolution MS (through different ionization sources, ESI and APCI) and the annotation strategies within MetaboScape 3.0 software allowed the identification of around 150 compounds in the profiles, showing great complementarity between the evaluated methodologies. The identified metabolites belonged to different chemical classes: triterpenic acids and dialcohols, tocopherols, sterols, free fatty acids, and several sub-types of phenolic compounds. The suitability of each platform and polarity (negative and positive) to determine each family of metabolites was evaluated in-depth, finding, for instance, that LC-ESI-MS (+) was the most efficient choice to ionize phenolic acids, secoiridoids, flavonoids and lignans and LC-APCI-MS was very appropriate for pentacyclic triterpenic acids (MS (-)) and sterols and tocopherols (MS (+)). Afterwards, a semi-quantitative comparison of the selected matrices was carried out, establishing their typical features (e.g., fruit skin was pointed out as the matrix with the highest relative amounts of phenolic acids, triterpenic compounds and hydroxylated fatty acids, and seed oil was distinctive for its high relative levels of acetoxypinoresinol and tocopherols).es_ES
dc.language.isoenges_ES
dc.publisherMDPIes_ES
dc.rightsAtribución 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es/*
dc.subjectOlea europaea L.es_ES
dc.subjectLiquid chromatographyes_ES
dc.subjectGas chromatography es_ES
dc.subjectMass spectrometry es_ES
dc.subjectSecondary metaboliteses_ES
dc.titleUnravelling the Distribution of Secondary Metabolites in Olea europaea L.: Exhaustive Characterization of Eight Olive-Tree Derived Matrices by Complementary Platformses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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