Unravelling the Distribution of Secondary Metabolites in Olea europaea L.: Exhaustive Characterization of Eight Olive-Tree Derived Matrices by Complementary Platforms
Metadatos
Mostrar el registro completo del ítemAutor
Olmo García, Lucía; Kessler, Nikolas; Neuweger, Heiko; Wendt, Karin; Olmo-Peinado, José María; Fernández Gutiérrez, Alberto; Baessmann, Carsten; Carrasco Pancorbo, AlegríaEditorial
MDPI
Materia
Olea europaea L. Liquid chromatography Gas chromatography Mass spectrometry Secondary metabolites
Fecha
2018-09-20Referencia bibliográfica
Olmo García, L. [et al.]. Unravelling the Distribution of Secondary Metabolites in Olea europaea L.: Exhaustive Characterization of Eight Olive-Tree Derived Matrices by Complementary Platforms. Molecules 2018, 23, 2419; doi:10.3390/molecules23102419.
Resumen
In order to understand the distribution of the main secondary metabolites found in
Olea europaea L., eight different samples (olive leaf, stem, seed, fruit skin and pulp, as well as
virgin olive oil, olive oil obtained from stoned and dehydrated fruits and olive seed oil) coming
from a Picudo cv. olive tree were analyzed. All the experimental conditions were selected so as
to assure the maximum coverage of the metabolome of the samples under study within a single
run. The use of LC and GC with high resolution MS (through different ionization sources, ESI and
APCI) and the annotation strategies within MetaboScape 3.0 software allowed the identification
of around 150 compounds in the profiles, showing great complementarity between the evaluated
methodologies. The identified metabolites belonged to different chemical classes: triterpenic acids
and dialcohols, tocopherols, sterols, free fatty acids, and several sub-types of phenolic compounds.
The suitability of each platform and polarity (negative and positive) to determine each family
of metabolites was evaluated in-depth, finding, for instance, that LC-ESI-MS (+) was the most
efficient choice to ionize phenolic acids, secoiridoids, flavonoids and lignans and LC-APCI-MS was
very appropriate for pentacyclic triterpenic acids (MS (-)) and sterols and tocopherols (MS (+)).
Afterwards, a semi-quantitative comparison of the selected matrices was carried out, establishing
their typical features (e.g., fruit skin was pointed out as the matrix with the highest relative amounts
of phenolic acids, triterpenic compounds and hydroxylated fatty acids, and seed oil was distinctive
for its high relative levels of acetoxypinoresinol and tocopherols).