Metallofluorescent Nanoparticles for Multimodal Applications

Delgado-Gonzalez, Antonio; García-Fernández, Emilio; Valero, Teresa; Cano-Cortés, Victoria; Ruedas Rama, María José; Unciti-Broceta, Asier; Sánchez Martín, Rosario María; Díaz Mochón, Juan José; Orte Gutiérrez, Ángel

doi:10.1021/acsomega.7b01984

Delgado Gonzalez_Metallofluorescent Nanoparticles (4.918Mb)

Identificadores

URI: http://hdl.handle.net/10481/51224

DOI: 10.1021/acsomega.7b01984

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Editorial

American Chemical Society

Date

2018-01-05

Referencia bibliográfica

Delgado-Gonzalez, Antonio; et. al. Metallofluorescent Nanoparticles for Multimodal Applications. ACS Omega 2018, 3, 144−153 [http://hdl.handle.net/10481/51224]

Sponsorship

This work was funded by the Consejeria de Economia, Innovacion, Ciencia y Empleo (Junta de Andalucia, grant BIO-1778), the Spanish Ministry of Economy and Competitiveness (grants BIO2016-80519-R and CTQ2014-56370-R) and the Fundación Ramón Areces. A.D.-G. acknowledges scholarships from the Spanish Ministry of Education (grant FPU14/02181) and the University of Granada, PhD programme in Biomedicine. T.V. thanks the Talentia Postdoc Fellowship Programme (grant 267226, co-funded by the Andalusian Knowledge Agency and the 7th Framework Program of the European Union). A.U.-B. is grateful to the EPSRC (EP/N021134/1) for funding. The authors thank the staff at the microscopy facility of the Centre for Integrative Biology (CIBIO) of the University of Trento (Italy), the staff at the electron microscopy facilities of the Centre for Scientific Instrumentation (CIC) of the University of Granada, and the staff at the Flow Cytometry unit at the Pfizer-University of Granada-Junta de Andalucia Centre for Genomics and Oncological Research (GENYO).

Abstract

Herein, we describe the synthesis and application of cross-linked polystyrene-based dual-function nano- and microparticles containing both fluorescent tags and metals. Despite containing a single dye, these particles exhibit a characteristic dual-band fluorescence emission. Moreover, these particles can be combined with different metal ions to obtain hybrid metallofluorescent particles. We demonstrate that these particles are easily nanofected into living cells, allowing them to be used for effective fingerprinting in multimodal fluorescence-based and mass spectrometry-based flow cytometry experiments. Likewise, the in situ reductions of the metal ions enable other potential uses of the particles as heterogeneous catalysts.

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