Metallofluorescent Nanoparticles for Multimodal Applications
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Delgado-Gonzalez, Antonio; García-Fernández, Emilio; Valero, Teresa; Cano-Cortés, Victoria; Ruedas Rama, María José; Unciti-Broceta, Asier; Sánchez Martín, Rosario María; Díaz Mochón, Juan José; Orte Gutiérrez, ÁngelEditorial
American Chemical Society
Date
2018-01-05Referencia bibliográfica
Delgado-Gonzalez, Antonio; et. al. Metallofluorescent Nanoparticles for Multimodal Applications. ACS Omega 2018, 3, 144−153 [http://hdl.handle.net/10481/51224]
Sponsorship
This work was funded by the Consejeria de Economia, Innovacion, Ciencia y Empleo (Junta de Andalucia, grant BIO-1778), the Spanish Ministry of Economy and Competitiveness (grants BIO2016-80519-R and CTQ2014-56370-R) and the Fundación Ramón Areces. A.D.-G. acknowledges scholarships from the Spanish Ministry of Education (grant FPU14/02181) and the University of Granada, PhD programme in Biomedicine. T.V. thanks the Talentia Postdoc Fellowship Programme (grant 267226, co-funded by the Andalusian Knowledge Agency and the 7th Framework Program of the European Union). A.U.-B. is grateful to the EPSRC (EP/N021134/1) for funding. The authors thank the staff at the microscopy facility of the Centre for Integrative Biology (CIBIO) of the University of Trento (Italy), the staff at the electron microscopy facilities of the Centre for Scientific Instrumentation (CIC) of the University of Granada, and the staff at the Flow Cytometry unit at the Pfizer-University of Granada-Junta de Andalucia Centre for Genomics and Oncological Research (GENYO).Abstract
Herein, we describe the synthesis and application
of cross-linked polystyrene-based dual-function nano- and
microparticles containing both fluorescent tags and metals.
Despite containing a single dye, these particles exhibit a
characteristic dual-band fluorescence emission. Moreover,
these particles can be combined with different metal ions to
obtain hybrid metallofluorescent particles. We demonstrate
that these particles are easily nanofected into living cells,
allowing them to be used for effective fingerprinting in
multimodal fluorescence-based and mass spectrometry-based
flow cytometry experiments. Likewise, the in situ reductions of
the metal ions enable other potential uses of the particles as
heterogeneous catalysts.