Improved reactive nanoparticles to treat dentin hypersensitivity.
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AuthorToledano Osorio, Manuel; Osorio Ruiz, María Estrella; Sánchez Aguilera, Fátima; Medina Castillo, Antonio Luis; Osorio Ruiz, Raquel; Toledano Pérez, Manuel
Toledano-Osorio M, Osorio E, Aguilera FS, Luis Medina-Castillo A, Toledano M, Osorio R. Improved reactive nanoparticles to treat dentin hypersensitivity. Acta Biomater. 2018 Mar 24. pii: S1742-7061(18)30164-8 [ http://hdl.handle.net/10481/50334]
SponsorshipThis work was supported by the Ministry of Economy and Competitiveness (MINECO) and European Regional Development Fund (FEDER) [Project MAT2017-85999-P]
The aim of this study was to evaluate the effectiveness of different nanoparticles-based solutions for dentin permeability reduction and to determine the viscoelastic performance of cervical dentin after their application. Four experimental nanoparticle solutions based on zinc, calcium or doxycycline-loaded polymeric nanoparticles (NPs) were applied on citric acid etched dentin, to facilitate the occlusion and the reduction of the fluid flow at the dentinal tubules. After 24 h and 7 d of storage, cervical dentin was evaluated for fluid filtration. Field emission scanning electron microscopy, energy dispersive analysis, AFM and Nano-DMA analysis were also performed. Complex, storage, loss modulus and tan delta (δ) were assessed. Doxycycline-loaded NPs impaired tubule occlusion and fluid flow reduction trough dentin. Tubules were 100% occluded in dentin treated with calcium-loaded NPs or zinc-loaded NPs, analyzed at 7 d. Dentin treated with both zinc-NPs and calcium-NPs attained the highest reduction of dentinal fluid flow. Moreover, when treating dentin with zinc-NPs, complex modulus values attained at intertubular and peritubular dentin were higher than those obtained after applying calcium-NPs. Zinc-NPs are then supposed to fasten active dentin remodeling, with increased maturity and high mechanical properties. Zinc-based nanoparticles are then proposed for effective dentin remineralization and tubular occlusion. Further research to finally prove for clinical benefits in patients with dentin hypersensitivity using Zn-doped nanoparticles is encouraged.