Maslinic Acid, a Triterpene from Olive, Affects the Antioxidant and Mitochondrial Status of B16F10 Melanoma Cells Grown under Stressful Conditions
Identificadores
URI: http://hdl.handle.net/10481/37239DOI: 10.1155/2015/272457
ISSN: 1741-427X
ISSN: 1741-4288
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Mokhtari, Khalida; Rufino Palomares, Eva; Pérez Jiménez, Amalia; Reyes Zurita, Fernando Jesús; Figuera, Celeny; García-Salguero, Leticia; Medina Vico, Pedro Pablo; Peragón Sánchez, Juan; Lupiáñez Cara, José AntonioEditorial
Hindawi Publishing Corporation
Materia
Maslinic acid (MA) Melanoma cancer Reactive oxygen species (ROS) Olive
Date
2015Referencia bibliográfica
Mokhtari, K.; et al. Maslinic Acid, a Triterpene from Olive, Affects the Antioxidant and Mitochondrial Status of B16F10 Melanoma Cells Grown under Stressful Conditions. Evidence-Based Complementary and Alternative Medicine, 2015: 272457 (2015). [http://hdl.handle.net/10481/37239]
Sponsorship
This study has been supported, in part, by funds of the consolidated Research Group BIO-157, from the General Secretariat of Universities, Research and Technology of the Ministry of Economy, Innovation, Science and Employment Government of the Junta de Andaluc´ıa (Spain), and by the Research Contract no. C-3650-00 under the program FEDER-INNTERCONECTA from the Spanish Government and European Union FEDER funds. Amalia P´erez-Jim´enez is a recipient of a postdoctoral research fellowship Torres- Quevedo no. PTQ 12-05739.Abstract
Maslinic acid (MA) is a natural compound whose structure corresponds to a pentacyclic triterpene. It is abundant in the cuticular
lipid layer of olives. MA has many biological and therapeutic properties related to health, including antitumor, anti-inflammatory,
antimicrobial, antiparasitic, antihypertensive, and antioxidant activities. However, no studies have been performed to understand
the molecular mechanism induced by this compound in melanoma cancer. The objective of this study was to examine the
effect of MA in melanoma (B16F10) cells grown in the presence or absence of fetal bovine serum (FBS). We performed cell
proliferation measurements, and the reactive oxygen species (ROS) measurements using dihydrorhodamine 123 (DHR 123) and
activities of catalase, glucose 6-phosphate dehydrogenase, glutathione S-transferase, and superoxide dismutase. These changes were
corroborated by expression assays. FBS absence reduced cell viability decreasing IC50 values of MA.The DHR 123 data showed an
increase in the ROS level in the absence of FBS. Furthermore, MA had an antioxidant effect at lower assayed levels measured as
DHR and antioxidant defense.However, at higher dosagesMAinduced cellular damage by apoptosis as seen in the results obtained.