@misc{10481/37239,
year = {2015},
url = {http://hdl.handle.net/10481/37239},
abstract = {Maslinic acid (MA) is a natural compound whose structure corresponds to a pentacyclic triterpene. It is abundant in the cuticular
lipid layer of olives. MA has many biological and therapeutic properties related to health, including antitumor, anti-inflammatory,
antimicrobial, antiparasitic, antihypertensive, and antioxidant activities. However, no studies have been performed to understand
the molecular mechanism induced by this compound in melanoma cancer. The objective of this study was to examine the
effect of MA in melanoma (B16F10) cells grown in the presence or absence of fetal bovine serum (FBS). We performed cell
proliferation measurements, and the reactive oxygen species (ROS) measurements using dihydrorhodamine 123 (DHR 123) and
activities of catalase, glucose 6-phosphate dehydrogenase, glutathione S-transferase, and superoxide dismutase. These changes were
corroborated by expression assays. FBS absence reduced cell viability decreasing IC50 values of MA.The DHR 123 data showed an
increase in the ROS level in the absence of FBS. Furthermore, MA had an antioxidant effect at lower assayed levels measured as
DHR and antioxidant defense.However, at higher dosagesMAinduced cellular damage by apoptosis as seen in the results obtained.},
organization = {This study has been supported, in part, by funds of the
consolidated Research Group BIO-157, from the General
Secretariat of Universities, Research and Technology of the
Ministry of Economy, Innovation, Science and Employment
Government of the Junta de Andaluc´ıa (Spain), and by
the Research Contract no. C-3650-00 under the program
FEDER-INNTERCONECTA from the Spanish Government
and European Union FEDER funds. Amalia P´erez-Jim´enez
is a recipient of a postdoctoral research fellowship Torres-
Quevedo no. PTQ 12-05739.},
publisher = {Hindawi Publishing Corporation},
keywords = {Maslinic acid (MA)},
keywords = {Melanoma cancer},
keywords = {Reactive oxygen species (ROS)},
keywords = {Olive},
title = {Maslinic Acid, a Triterpene from Olive, Affects the Antioxidant and Mitochondrial Status of B16F10 Melanoma Cells Grown under Stressful Conditions},
doi = {10.1155/2015/272457},
author = {Mokhtari, Khalida and Rufino Palomares, Eva and Pérez Jiménez, Amalia and Reyes Zurita, Fernando Jesús and Figuera, Celeny and García-Salguero, Leticia and Medina Vico, Pedro Pablo and Peragón Sánchez, Juan and Lupiáñez Cara, José Antonio},
}