Maslinic Acid, a Triterpene from Olive, Affects the Antioxidant and Mitochondrial Status of B16F10 Melanoma Cells Grown under Stressful Conditions Mokhtari, Khalida Rufino Palomares, Eva Pérez Jiménez, Amalia Reyes Zurita, Fernando Jesús Figuera, Celeny García-Salguero, Leticia Medina Vico, Pedro Pablo Peragón Sánchez, Juan Lupiáñez Cara, José Antonio Maslinic acid (MA) Melanoma cancer Reactive oxygen species (ROS) Olive Maslinic acid (MA) is a natural compound whose structure corresponds to a pentacyclic triterpene. It is abundant in the cuticular lipid layer of olives. MA has many biological and therapeutic properties related to health, including antitumor, anti-inflammatory, antimicrobial, antiparasitic, antihypertensive, and antioxidant activities. However, no studies have been performed to understand the molecular mechanism induced by this compound in melanoma cancer. The objective of this study was to examine the effect of MA in melanoma (B16F10) cells grown in the presence or absence of fetal bovine serum (FBS). We performed cell proliferation measurements, and the reactive oxygen species (ROS) measurements using dihydrorhodamine 123 (DHR 123) and activities of catalase, glucose 6-phosphate dehydrogenase, glutathione S-transferase, and superoxide dismutase. These changes were corroborated by expression assays. FBS absence reduced cell viability decreasing IC50 values of MA.The DHR 123 data showed an increase in the ROS level in the absence of FBS. Furthermore, MA had an antioxidant effect at lower assayed levels measured as DHR and antioxidant defense.However, at higher dosagesMAinduced cellular damage by apoptosis as seen in the results obtained. 2015-09-03T11:18:38Z 2015-09-03T11:18:38Z 2015 journal article Mokhtari, K.; et al. Maslinic Acid, a Triterpene from Olive, Affects the Antioxidant and Mitochondrial Status of B16F10 Melanoma Cells Grown under Stressful Conditions. Evidence-Based Complementary and Alternative Medicine, 2015: 272457 (2015). [http://hdl.handle.net/10481/37239] 1741-427X 1741-4288 http://hdl.handle.net/10481/37239 10.1155/2015/272457 eng http://creativecommons.org/licenses/by-nc-nd/3.0/ open access Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License Hindawi Publishing Corporation